醋酸杆菌Acetobacter xylinum pde基因敲除研究  

Studying on the pde-gene′s disruption of the Acetobacter xylinum

作　　者：张雯[1] 齐香君[1] 李彦军[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2013年第5期121-125,共5页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅自然科学基金项目(2012JM2005);陕西省教育厅科研计划项目(12JK0954)

摘　　要：基于磷酸二酯酶(PDE)对细菌纤维素(BC)合成产生抑制的机理,对细菌纤维素生产菌株醋酸杆菌(Acetobacter xylinum)的PDE编码基因(pde基因)进行了基因敲除研究,构建PDE失活型(PDE-)重组菌株,并通过氨苄青霉素及氯霉素抗生素培养基筛选出目的重组菌株.发酵及遗传稳定性实验结果表明,PDE-重组菌株发酵生产BC产量比出发菌株最大可提高38%,且遗传性能稳定.本研究为提高BC发酵产量奠定了基础.According to the mechanism that the phosphodiesterase（PDE） would restrain the synthesis of bacterial cellulose（BC）, pde-gene which code PDE in the Acetobacter xylinum producing BC is disrupted, and new strains named PDE strain whose PDE have non-activity are constructed. The aim strains are filtrated using the culture medium which contains ampi- cillin and chloromycetin. The results of the fermentation shows that the BC output of PDE strain can increased by 38% than the starting strain, and its heredity was stable. This study has laid certain foundation to increase the fermentation output of BC.

关 键 词：细菌纤维素 基因敲除 磷酸二酯酶 

分 类 号：Q812[生物学—生物工程]