土壤中1株解磷细菌的筛选及其生长特性的研究  

A phosphorus bacteria separated from soil and its growth characteristics

作　　者：原海兵[1] 刘军[1] 韩志双[1] 郇阿梅[1] 石娇娇[1] 

机构地区：[1]四川理工学院生物工程系,自贡643000

出　　处：《安徽农业大学学报》2013年第5期843-848,共6页Journal of Anhui Agricultural University

基　　金：四川省科技厅重点项目(2013SZ0096);宜宾市科技局重点项目(2012SF033)共同资助

摘　　要：从四川省自贡市施用磷肥较多的土壤中取样，通过平板法和检测液体培养后的可溶性磷含量，筛选出1株高效解磷细菌L-p1，对菌株进行生理生化特征的鉴定和16SrDNA区段序列分析，并对菌株的生长特性进行了研究。结果表明，筛选到的解磷细菌L-P1是一株巨大芽孢杆菌（Bacillus megateriumstrain），其分解磷矿粉的能力为5．3ktg·mg-1，比对照组高60．6％。它对糖源的利用能力为：甘露醇〉蔗糖〉葡萄糖〉淀粉〉乳糖；对碳源的利用能力为蔗糖〉甘露醇〉葡萄糖〉淀粉〉乳糖；对氮源的利用能力为牛肉膏〉硫酸铵〉蛋白胨〉硝酸钾〉尿素。磷细菌的最适生长温度为28～32＊（2，最适pH生长范围为7～8，培养28h菌体生物量达到最大。A phosphorus bacteria named L-P1 was separated in enriched phosphate soil from Zigong, Sichuan Province by plate method and detecting the available phosphorus in the culture, and then the strain was identified through biochemical characteristics and 16SrDNA sequence alignment. Its growth characteristics were also stud- ied. The results showed that the bacterium is a Bacillus megaterium strain, and phosphate-dissolving ability was 5.3 μg.mg-1, which was 60.6% higher than the control. And the ability to use sugar source was ranked as： Manni- tol〉sucrose〉glucose〉starch〉lactose; the ability to use carbon source was ranked as： Sucrose〉 mannitol〉 glu- cose〉starch〉lactose; the ability to use nitrogen source was ranked as： Beef extract〉ammonium sulfate〉 peptone〉 urea〉potassium nitrate. The optimal condition for cultivation of L-P1 strain was at 28-32℃ and pH 7-8. Under this condition, the biomass of L-P1 could reach the maximum after culture for 28 h.

关 键 词：解磷细菌 解磷能力 16SrDNA 生长特性 

分 类 号：Q939.1[生物学—微生物学]