柏木木屑中精油成分分析及抑菌抗氧化活性研究  

Chemical Composition,Antibacterial and Antioxidant Activities of Essential Oil fromCupressus funebris Chips

作　　者：侯仰帅[1] 姜媛媛[1] 晏明[1] 余静[1] 张利[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《四川农业大学学报》2013年第3期314-318,共5页Journal of Sichuan Agricultural University

基　　金：四川农业大学大学生创新性实验计划项目(111062633);四川省科技厅科技支撑计划项目(2011ZO0006)

摘　　要：【目的】以柏木木屑为原料进行柏木精油的提取,实现柏木资源的最大化利用和可持续发展。【方法】采用水蒸气蒸馏法提取柏木精油,通过气相色谱-质谱联用技术(GC-MS)分析精油成分,采用滤纸片扩散法测定精油和链霉素分别单独使用以及两者联合使用时对供试细菌的抑制情况,并测定了精油的总还原力以及对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟基自由基(·OH)、超氧阴离子自由基(O-2·)的清除能力。【结果】结果表明:精油主要成分为柏木醇(26.06%)、罗汉柏烯(18.25%)、α-柏木烯(15.06%)、(+)-花侧柏烯(8.23%)、α-姜黄烯(3.20%)、i-石竹烯(2.83%)、β-雪松烯(2.69%)和(+)-β-柏木烯(2.48%)等。柏木精油单独使用时对沙门氏菌的抑菌效果属高度敏感,对其他供试菌的抑菌效果属中度敏感。在大肠杆菌、枯草芽孢杆菌、霍氏肠杆菌、克雷伯氏菌、炭疽杆菌的抑菌实验中,链霉素起主要作用;而在沙门氏菌、枯草芽孢杆菌抑菌实验中,柏木精油起主要作用。柏木精油的抗氧化活性与精油浓度呈现一定的量效关系,具有较强的还原力。对DPPH·、·OH、O-2·的IC50(清除率达到50%时的质量浓度)分别为2.55、2.23和1.30mg/mL,表现出较强的抗氧化活性。【结论】柏木木屑精油具有较强的抑菌能力以及抗氧化能力,为生物杀菌剂以及天然食品防腐剂和抗氧化剂的研究提供了理论依据。[Objective] Essential oil in C. funebris chips was extracted and optimizeresource utiliza- tion and sustainable development of C. funebris can be realized. [Methodl The essential oil was extracted by steam distillation and oil components were separated and identified by gas chromatog- raphy-mass spectrum （GC-MS）. The antibacterial effects were investigated using filter paper dif fusion methods when essential oil and streptomycin used alone and in combined with each other. The reducing power and scavenging capacity against 1,1-streptomycin, Diphenyl-2-picrylhydrazyl and hydroxyl and superoxide anion free radical antioxidant activities were measured. [Resultsl The essential oil of C. funebris mainly contained cedrol （26.06%）, thujopsene （18.25%）, cu parene （15.06%）, a-cedrene （8.23%）, a-curcumene （3.20%）, β-Caryophyllene （2.83%）, β-Hi- machalene （2.69%） and （＋）-β-Cedrene （2. 48%）. Essential oil alone was highly sensitive to Salmonella and moderately sensitive to the other tested strains. In Escherichia coli, Enterobacter hormaechei, Bacillus anthraci and Klebsiella antibacterial experiments, streptomycin play a major role. While in Salmonella and Bacillus subtilis bacteria experiments, essential oils play a major effect. The antioxidant activity of essential oil increased with the increase in concentration, ex- hibiting stronger reducing power. The half maximal inhibitory concentrations （ICs0） against 1,1- Diphenyl-2-picrylhydrazyl, hydroxyl and superoxide anion free radical were 2.55, 2.23 and 1.30 mg/mL, respectively, indicating stronger antioxidant activity. [Conclusion] The essential oil from C. funebris has strong antibacterial and antioxidant capacity, which provides a theoretical basis for the study of biological fungicides and natural food preservatives and antioxidants.

关 键 词：柏木木屑 柏木精油 成分 抑菌活性 抗氧化活性 

分 类 号：Q946.33[生物学—植物学]