8种傣药提取物体外抑制α-糖苷酶活性的研究  

Research of the α-glucosidase Inhibition Activity of the Extracts from 8 Dai Nationality Medicines in vitro

作　　者：胡鑫[1] 齐艳菲[1] 王兴云[1] 盘妮[1] 刘丽娜[1] 王丽[1] 王德彬[1] 徐婧[1] 杨光忠[1] 

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2013年第3期60-62,117,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学科研基金资助项目(KYCX110507Z)

摘　　要：采用α-葡萄糖苷酶活性抑制酶学模型,以96微孔板法测定了8种具有降糖作用傣药的石油醚、正丁醇、乙酸乙酯、水提取物体外对α-葡萄糖苷酶的抑制作用,筛选具有葡萄糖苷酶抑制作用的有效部位.结果表明:8种傣药的提取物均有α-葡萄糖苷酶抑制活性,抑制作用强度与其浓度呈正相关.其中,黑皮跌打藤茎,其正丁醇提取物活性最高,IC50=17.74μg/mL,显著优于对照药物阿卡波糖.To screen the active extracts with the inhibitory effects to α-glucosidase, eight hypoglycemic Dai Nationality Medicine were extracted by petroleum ether, ethyl acetate, n-butyl alcohol and water respectively. 96-Pore plate method was used to establish the enzymatic model for the determination of the inhibitory effect. The results indicated that the extracts from the eight Dai Nationality Medicines all had inhibitory effects to α-glucosidase in proportion to their concentrations. Among those the n-butyl alcohol extract from Fissistigma polyanthum showed the highest inhibitory activity with an IC50 of 17.74 μg/mL, significantly better than the control medicine Acarbose.

关 键 词：Α-葡萄糖苷酶 抑制活性 黑皮跌打藤茎 

分 类 号：TQ460[化学工程—制药化工] Q55[生物学—生物化学]