中药红曲基原真菌的酯酶同工酶分析  

ANALYSIS OF ESTERASE ISOENZYME OF THE ORIGIN FUNGI OF THE CHINESE TRADITIONAL MEDICINE HONGQU

作　　者：邢旺兴[1] 宓鹤鸣[1] 陈士景[2] 程荣珍[3] 

机构地区：[1]第二军医大学药学院药物分析教研室,上海200433 [2]解放军117医院药剂科,杭州310013 [3]杭州尖峰德康药业有限公司,杭州310023

出　　处：《微生物学通报》2000年第6期437-440,共4页Microbiology China

基　　金：解放军95’重点课题基金!(No.96-Z-059)

摘　　要：探讨中药红曲基原真菌的同工酶分析方法。用凝胶电泳法分析７种红曲霉菌的胞内和胞外酯酶同工酶，模糊聚类法分析供试菌间的亲缘关系。红曲霉的酯酶同工酶胞内酶明显多于胞外酶，种间存在明显差异。同工酶分析可以作为红曲霉菌传统分类的重要辅助手段。Establish a analysis method of isoenzyme to classification of origin fungi of the Chinese traditional medicine, Hongqu. Established a gel electrophoresis method to analysis of esterase isoenzyme of 7 ordinary species Monascus and analyses the phylogenetic relationship by clustering analysis. The number of bands of intracelluar isoenzyme of Monascus are more than that of extracellular isoenzyme. There exist obviously differences between species. The analysis of isocnzyme is a important secondary technique being able to using to classify and identify of Monascus fungi.

关 键 词：红曲 红曲霉菌 同工酶 分类 

分 类 号：Q936[生物学—微生物学]