pH反馈补料方法在γ-聚谷氨酸发酵中的运用  

Application of pH feedback-controlled substrate feeding method inγ-polyglutamic acid fermentation

作　　者：乔长晟[1] 兰玲峰[1] 张帅[1] 李政[2] 郝华璇 

机构地区：[1]天津科技大学生物工程学院工业微生物重点实验室,天津300457 [2]天津工业大学纺织学院,天津300387 [3]天津北洋百川生物技术有限公司,天津300457

出　　处：《工业微生物》2013年第5期39-43,共5页Industrial Microbiology

基　　金：天津市科委科技支撑计划(No.09ZCKFSH00800)

摘　　要：建立了一种基于发酵液pH反馈控制γ-聚谷氨酸发酵中葡萄糖补加的方法,即:发酵过程中pH再次回升至7.0时,补加浓缩葡萄糖使发酵液中残糖达到1.5 g/L时停止补加。结果表明,利用该补料方法可较理想地将发酵液中葡萄糖浓度控制在1.0 g/L,发酵终产量达到42.5g/L,产率提高了46.8%,谷氨酸转化率提高了17.7%。A pH feed-back-controlled fed-batch culture method was proposed for the production of γ-polyglutamic acid. When the pH was ascended to 7.0 again, substrate feeding was started ; and when the glucose concentration reached to 1. 5 g/L, the feeding was stopped. The result indicated that, the glucose concentration in the fermentation broth was maintained at 1.0 g/L, ideally. At the end of fermentation, 42.5 g/L γ-polyglutamic acid was produced. The γ-PGA productivity and glutamate conversion rate increased by 46.8% and 17.7% , respectively.

关 键 词：pH反馈 1-聚谷氨酸 分批补料发酵 地衣芽孢杆菌CGMCC3336 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]