响应面优化乳杆菌PC-3菌株产共轭亚油酸的工艺研究  

Optimization of Medium Composition and Culture Conditions for Conversion of Linoleic Acid to Conjugated Linoleic Acid by Lactobacillus PC-3

作　　者：章立新[1] 王武[1] 吴巧[1] 董琪[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2013年第19期213-218,共6页Food Science

基　　金：安徽省年度重点科研项目(12070403077)

摘　　要：以共轭亚油酸(CLA)转化率为考察指标,研究乳杆菌PC-3菌株转化生产CLA的发酵工艺。在单因素试验中考察培养基种类、乳糖与硫酸铵添加量比、菌液接种量、培养时间、亚油酸添加量、吐温-80添加量对CLA转化率影响的基础上,采用三因素三水平响应面试验设计优化乳杆菌PC-3菌株产共轭亚油酸的工艺条件。结果表明:乳杆菌PC-3菌株产共轭亚油酸的较佳条件为:诱导培养基为MRS培养基、乳糖与硫酸铵添加量比3:2、接种量3.0%、培养时间43.0h、亚油酸(LA)添加量(体积分数)1.30‰、乳化剂吐温-80添加量2.00g/L,此条件下CLA转化率达到6.87%,理论值可达6.96%,实验值与理论预测值的相对误差为1.29%。The medium composition and culture conditions for conversion of linoleic acid （LA） to conjugated linoleic acid （CLA） by Lactobacillus PC-3 were optimized by response surface methodology to obtain maximum conversion efficiency of CLA. Culture medium type, lactose/ammonium sulfate concentration ratio, inoculums amount, culture time, LA concentration and Tween-80 concentration were investigated by one-factor-at-a-time experiments. A three-variable, three-level response surface design was used to optimize inoculum amount, culture time and LA concentration. The results showed that the optimal conversion conditions of LA was MRS medium, inoculums amount of 3.0%, culture time of 43.0 h, LA concentration of 1.30‰ and Tween-80 concentration of 2.00 g/L. Under the optimized conditions, the experimental conversion efficiency of CLA was 6.87% compared to 6.96% from theoretical predictions, showing a relative error of 1.29% between both.

关 键 词：共轭亚油酸 乳杆菌PC-3 响应面分析法 优化 

分 类 号：Q815[生物学—生物工程]