担载不同结构胺基的P(GMA-EDGMA)大孔载体的固定化酶活  

Immobilization of Enzyme on P(GMA-EDGMA)Macroporous Carriers Modified by Different Amines

作　　者：厉向杰[1] 张宝亮[1] 贾佳[1] 张秋禹[1] 

机构地区：[1]西北工业大学理学院,陕西西安710072

出　　处：《高分子材料科学与工程》2013年第10期89-93,共5页Polymer Materials Science & Engineering

基　　金：国家自然科学基金资助项目(51173146);航天科技创新基金重点项目(CASC201106);西北工业大学基础研究基金(JC20120248)

摘　　要：设计并制备了一种树枝状超支化胺(DHA-NH2),并用红外光谱(IR)、核磁共振谱(NMR)对其结构和性能进行了研究。利用DHA-NH2、乙二胺、己二胺、二乙烯三胺分别修饰P(GMA-EDGMA)树脂,制备得到4种表面带有不同胺基结构的固定化酶载体,以木瓜蛋白酶为被固定酶模型,研究表面胺基结构与固定化酶活之间的构效关系。结果表明,在胺基含量基本相同的条件下,载体固定化酶活顺序为二乙烯三胺>己二胺>乙二胺>DHA-NH2;活性链段数目、活性链段官能团密度以及活性链段长度共同决定固定化酶的酶活,且增加活性链段数目、增大活性链段官能团密度以及增长活性链段长度均能在一定程度上提高固定化酶活。Hyperbranched amine （DHA-NHz） with dendritic structure was designed and synthesized. Infrared spectrum and nuclear magnetic resonance were employed to investigate the structure and property. P （GMA- EDGMA） resins were modified by DHA-NHz, ethylenediamine, hexamethylenediamine and diethylenetriamine respectively, then four immobilized enzyme carriers of which surfaces has different amino groups were obtained. Papain was chosen as the model of immobilized enzyme to study the structure-activity relationship between anaino structures and immobilized enzyme activity. The results indicate that when the amino contents are almost the same, the activity sequence for immobilization of papain is diethylenetriamine〉 hexamethylenediamine 〉 ethylenediamine 〉 DHA-NH2. The activity of immobilized enzyme was decided by the number, density of functional groups and length of active segments. Any one of these three factors increases, the immobilized enzyme activity can be improved in a certain content.

关 键 词：胺基修饰 大孔树脂 固定化酶 木瓜蛋白酶 酶活性 

分 类 号：Q814.2[生物学—生物工程]