四类食品中甜蜜素检测方法的改进  

Modified methond for detection of sodium cyclamate in four kinds of foods

作　　者：陈静文[1] 薛亚东[2] 包蕾[2] 张轶群[2] 

机构地区：[1]昆明理工大学环境科学与工程学院,云南昆明650500 [2]玉溪市疾病预防控制中心,云南玉溪653100

出　　处：《中国热带医学》2013年第9期1064-1066,共3页China Tropical Medicine

摘　　要：目的建立毛细管柱气相色谱法分析四类食品中的甜蜜素含量。方法样品中的甜蜜素经溶剂萃取、NaNO2和H2SO4衍生化,衍生产物用HP-5毛细管柱气相色谱法进行测定。结果在0.0～1000.0μg/ml的线性范围内,甜蜜素含量呈良好线性关系(相关系数0.99965),样品测定的相对标准偏差为1.78%～3.87%(n=6),平均回收率在95.1%～106.0%。结论本方法简便,快速,准确,适用于四类食品的测定。Objective To establish the method for determination of sodium cyclamate in four kinds of foods by capillary gas chromatography. Methods Sodium cyclamate in the samles was extracted and derivatized by NaNO2 and H2SO4.. then determined by HP-5 capillary gas chromatography. Results Sodium cyclamate content was in a good linear relation （r=- 0.99965） with the range from 0.0 - 1000.0μg/mL, the RSDs were from 1.78% to 3.87%（n=6）,and the average recovery rates were from 95.1% to 106.0%. Conclusion This method is simple, rapid, accurate and suitable for determination of sodium cyclamate in four kinds of foods.

关 键 词：甜蜜素 毛细管气相色谱 前处理 改进 

分 类 号：R155.5[医药卫生—营养与食品卫生学]