石榴皮多酚对脂变L-02肝细胞HMG-CoA还原酶mRNA表达的影响  

Effects of Pomegranate Peel Polyphenols(PPPs) on the Expression of HMG-CoA Reductase mRNA of Human L-02 Hepatocyte

作　　者：梁俊[1] 李建科[1] 刘永峰[1] 张华峰[1] 马倩倩[1] 赵伟[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062

出　　处：《食品与生物技术学报》2013年第9期957-961,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31171677)

摘　　要：研究了石榴皮多酚纯化物、安石榴苷和石榴鞣花酸对脂变L-02肝细胞胆固醇合成限速酶HMG-CoA还原酶mRNA表达的影响;采用MTT法筛选石榴皮多酚适宜浓度;体积分数50%胎牛血清的RPMI-1640培养基与L-02肝细胞孵育48 h建立脂肪变性肝细胞模型;实验分为正常组、模型组和治疗组,治疗组加入不同浓度的受施物,继续培养48 h,采用RT-PCR法检测不同受施物对脂变L-02肝细胞HMG-CoA还原酶mRNA表达的影响;结果显示:石榴皮多酚均能呈剂量依赖性地减弱脂变L-02肝细胞mRNA表达,且以100μg/mL的安石榴苷标品抑制作用最强;表明石榴皮多酚降肝细胞总胆固醇作用是通过降低HMG-CoA还原酶mRNA表达实现的,安石榴苷是石榴皮多酚中降血脂的主要活性形式。The objective of this manuscript is to study the effects of Pomegranate Peel Polyphenols, Punicalagine and ellagic acid on the expression of HMG-CoA reductase mRNA of human L-02 hepatocyte. The model of steatotic L-02 hepatocyte was firstly established by cultured with 50% （V/ V） fetal bovine serum for 48 hours, and the appropriate concentrations of PPPs were detected by MTF assay. Then the experiments were divided into three groups：normal group,model group and therapy group,different contents of PPPs is added to the therapy group. After 48 h cultured,the mRNA expression level of HMG-CoA reductase was detected by RT-PCR after 48 h cultured. It was found that the HMG-CoA reductase mRNA expression level was significantly inhibited by PPPs treatment groups ,especially for the case of 100 μg/mL Punicalagin group. From the aboved results,the possible mechanisms of PPPs decreasing the intraeellur cholestol are related to downregulation of HMG-CoA reductase mRNA＇s expression ,Punicalagin was the key component in PPPs on lipid-lowering.

关 键 词：石榴皮多酚 安石榴苷 石榴鞣花酸 L-02肝细胞 HMG-COA还原酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]