子宫内膜腺癌P53蛋白与基因突变的研究  

P53 overexpression and mutation in endometrial carcinoma

作　　者：何畏[1] 魏丽惠[2] 卞度宏[1] 王建六[2] 富琪[2] 虞幸[2] 

机构地区：[1]重庆医科大学临床学院妇产科,重庆400016 [2]北京医科大学人民医院妇产科,北京100038

出　　处：《重庆医科大学学报》2000年第4期362-364,共3页Journal of Chongqing Medical University

基　　金：美国中华医学会奖学金项目!(北医奖学金第022号)

摘　　要：目的　研究p53基因突变对子宫内膜腺癌临床病理特点及转归的影响。方法　运用免疫组化染色和聚合酶联反应-单链构象多态性分析（PCR-SSCP），检测子宫内膜腺癌患者P53的蛋白表达与基因突变。结果　子宫内膜腺癌患者P53蛋白表达阳性率为34.15%，阳性者5年存活率明显低于阴性者（Ｐ =0.037），核过表达仅与组织分化相关，而与患者年龄、分期、浸肌程度、淋巴结转移、附件转移均无关（Ｐ> 0.05）；肿瘤患者p53突变率23.08%，且集中在外显子7、5、6；两法的一致率高（76.92%）。结论　P53突变与子宫内膜癌组织分化有关，与临床预后有关。：Objective To investigate p53 gene mutations in endometrial carcinoma（EC）,and their correlation with clinical features and prognosis. Methods Using immunohistochemical staining（IMH）and polymerase chain reaction-single strand conformation polymorphism（PCR-SSCP）methods, examine the protein expression and gene mutation of p53 in EC. Results The general positive rate of P53 protein was 34.15%. The expression of p53 was not correlated with age，clinical stage，myometrial invasion ，lymph node or ovarian metastasis（Ｐ>0.05），but the pathologic grade（Ｐ<0.05）.Survival curves determined by Kaplan-Meier method showed that p53 overexpression was associated with a poor prognosis in EC（Ｐ<0.05）.The positive rates of p53 gene mutation was 23.08% in EC, and the mutation were located on exon 5，6，and 7.The concordance rate of IMH and PCR-SSCP was 76.92%. Conclusion The mutation of p53 gene were associated with the pathologic grade and poor prognosis in EC.

关 键 词：子宫内膜腺癌 基因突变 P53蛋白 

分 类 号：R737.330.2[医药卫生—肿瘤]