乳铁蛋白在人脑胶质瘤中的表达及意义  

The Expressions and Significance of Lactoferrin in Human Gliomas

作　　者：周振海[1] 方加胜[1] 谭泽明[1] 张明宇[1] 王延金[1] 

机构地区：[1]中南大学湘雅医院神经外科,湖南长沙410000

出　　处：《现代生物医学进展》2013年第25期4801-4804,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30600636)

摘　　要：目的:通过对乳铁蛋白生物学特性的研究,分析其在人脑胶质瘤中的表达及意义。方法:通过免疫组化方法测定10例非肿瘤脑组织和46例胶质瘤(其中低级别胶质瘤27例(Ⅰ级和Ⅱ级)和高级别胶质瘤19例(Ⅲ级和Ⅳ级)中乳铁蛋白的表达情况并分析二者的相关性。用RT-PCR技术及Western-blot方法测定非肿瘤脑组织、低级别组胶质瘤和高级别胶质瘤组乳铁蛋白的表达量并分析二者的相关性。结果:乳铁蛋白在非肿瘤脑组织及不同级别胶质瘤中的表达情况不同,其在低级别胶质瘤中有表达,略低于非肿瘤脑组织;而在高级别胶质瘤中表达显著减少或者表达不明显,明显低于低级别胶质瘤和非肿瘤脑组织(免疫组化、RT-PCR及Western-Blot的P值分别为P=0.001、P=0.003、P=0.004)。结论:乳铁蛋白在非肿瘤脑组织和不同级别胶质瘤中的表达有差异,提示乳铁蛋白参与了人脑胶质瘤发生与发展的过程。高级别胶质瘤的高度恶性和高侵袭性可能与乳铁蛋白表达的下调从而丧失对肿瘤细胞的抑制作用相关。Objective： To investigate the expression and significance of lactoferrin in human gliomas. Methods： The expression of lactoferrin were detected by immunohistochemical, RT-PCR and Western-Blot methods in 10 case nomal brain tissues and 46 cases of hu- man glioma that contains 27 cases of grade I -grade II and 19 cases of grade III-grade 1V. Results： The expression oflactoferrin is dif- ferent in nomal brain tissue and glioma, even in different grade in glioma. Lactoferrin expresses in grade I -grade II glioma was lower than nomal brain tissues, and there is no positive expression or obvious decrease in grade III-grade IV. The positive expression rate of lactoferrin in grade III-grade IV gliomas was significant lower than that in nomal brain tissues and grade I -grade II gliomas. Con- clusion： The expression of lactoferrin is different in nomal brain tissues and different grade gliomas. It reveals that lactoferrin played an important role in glioma.

关 键 词：乳铁蛋白 胶质瘤 免疫组化 RT—PCR Western—Blot 

分 类 号：R739.4[医药卫生—肿瘤]