GA_3打破长白楤木(Aralia continentalis)种子休眠过程中的糖代谢分析  

Effect of Gibberellin(CA_3) on Carbohydrate Metabolism of Aralia continentalis Seeds in the Dormancy-breaking Process

作　　者：范文丽[1] 李天来[1] 冯颖[1] 

机构地区：[1]沈阳农业大学设施园艺省部共建教育部重点实验室,沈阳110161

出　　处：《沈阳农业大学学报》2013年第5期708-711,共4页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(31000803);辽宁省农业攻关项目(2011215003)

摘　　要：药食兼用野生蔬菜长白楤木(Aralia continentalis)种子具有休眠特性,在25℃/5℃变温条件下对长白楤木子在休眠解除过程中淀粉酶活性、淀粉、还原糖、蔗糖含量变化,以及种子萌发率与淀粉酶含量及糖含量进行相关分析。结果表明,GA3能够促进淀粉酶活性增加,淀粉含量、还原糖含量均随处理时间的延长而增加,为种子休眠解除提供了必要的物质和能量。α-淀粉酶活性与淀粉含量、还原糖含量呈显著正相关,而与蔗糖含量呈负相关,说明GA3能够促进种子休眠解除过程的分解代谢与合成代谢,可有效解除长白楤木种子休眠,150mg·L-1GA3处理30d种子的萌发率可达52.4%。Aralia continentalis Kitagwa seeds have the characteristic of dormancy. The changes of the activities of Amylase and the contents of metabolic substances in the A. continentalis seed dormancy-breaking process were invesligatel under fluctuating temperature stratification, and the correlation of the germination rate with sugar contents was analyzed. Results showed that： The a-amylase activity and the reducing sugar content,starch content in the seed increased significantly after using GA3 under alternating temperature, enough substance and energy were supplied to break seed dormancy. Positive correlation between α- Amylase activity and starch content, reducing sugar content was negative correlation was between α-Amylase activity and sucrose, whick indicates GA3 accelerated the substance catabolism and anabolism during seeds breaking dormancy. Therefores, GA3 played a vital role in breaking the dormancy of the seed, the germination rate reached 52.4% under 150mg.L-1GA3 treatment in 30d. Key words： Ardia continentalis seed; dormancy; GA3 ; carbohydrate metabolism;or-amylase activity; the reducing sugar content; sucrose content; starch content

关 键 词：长白楤木种子 休眠 赤霉素 糖代谢 淀粉酶活性 还原糖 蔗糖 淀粉 

分 类 号：S665.9[农业科学—果树学] Q949.763.2[农业科学—园艺学]