芝麻枯萎病生防细菌的筛选  

Screening of biocontrol bacteria against sesame Fusarium Wilt

作　　者：王俊芳[1,2] 王淼[1] 张颖[1] 王刚[1,2] 

机构地区：[1]河南大学生命科学学院,河南开封475004 [2]河南大学生物工程研究所,河南开封475004

出　　处：《广东农业科学》2013年第19期72-74,共3页Guangdong Agricultural Sciences

基　　金：河南省教育厅项目(2011B210001)

摘　　要：从采自河南东部地区的36份健康芝麻样品中共分离出421株内生细菌。利用KBM、PDA和MYG等培养基,通过平板对峙培养法测定了421株芝麻根部内生细菌对尖孢镰刀菌(Fusarium oxysporium)的拮抗作用,结果显示:有3株菌(A21,G20,G36)拮抗性显著,室内防效分别为52%、40%、67%;通过对其产酶能力的检测,3个菌株具有产不同细胞壁降解酶的能力,其可能具有不同的生防作用机制。通过对拮抗性菌株生理生化特征的初步鉴定,可知G36为芽孢杆菌属(Bacillus.sp.)。The study isolated 421 endophytic bacteria from 36 samples of healthy sesame roots growing in eastern Henan province. These bacterial strains were isolated on King＇s B medium （KBM）, incubated on King＇s B medium （KBM）, potato dextrose agar （PDA） and malt yeast glucose agar （MYG）. Antagonistic activity of these bacteria against Fusarium oxysporium was tested by dural culture method. The result showed that 3 bacterial strains （A21, G20, G36） had significant antagonistic activity, and the biological control efficacies were 52%, 40%, 67% respectively. The detection of the enzyme production ability indicated that the 3 bacterial strains produced different cell wall degrading enzymes, and possibly had different bio-control mechanism. The test of physiological and biochemical characteristics of endophytic bacteria indicated that G36 belonged to Bacillus sp.

关 键 词：内生细菌 芝麻 尖孢镰刀菌 拮抗 

分 类 号：S482.29[农业科学—农药学]