植物酵素急性毒性与亚慢性毒性的研究  

Studies on the Acute and Subchronic Toxicities of Plant Enzyme Product

作　　者：赵金凤[1,2] 石永伟 曲佳乐 皮子凤[1] 崔玉东[2] 宋凤瑞[1] 刘志强[1] 

机构地区：[1]中国科学院长春应用化学研究所,吉林长春130022 [2]黑龙江八一农垦大学生命学院,黑龙江大庆163000 [3]吉林敖东大高酵素有限公司,吉林延边133700

出　　处：《食品与发酵科技》2013年第5期13-17,共5页Food and Fermentation Science & Technology

摘　　要：目的:通过急性毒性试验和亚慢性毒性试验研究植物酵素的安全性。方法:急性毒性试验以小鼠为研究对象,测定小鼠经口灌胃给予植物酵素的最大耐受剂量;亚慢性毒性试验以SD大鼠为研究对象,大鼠经口灌胃给予植物酵素90d后,观察对大鼠一般状况,肝、肾功能及脏器病理变化的影响。结果显示,急性毒性试验小鼠最大耐受剂量超过108mL/(kg·BW),相当于人体推荐口服剂量60mL/(人·d)的108倍;亚慢性毒性试验高、中、低剂量下,大鼠一般状况良好,正常生长,未出现中毒症状,无死亡,脏器系数及组织学检查无异常,对大鼠肝脏和肾脏无毒性作用。结果表明,植物酵素一日内给小鼠大量灌胃,无急性毒性反应,亚慢性毒性。The safety of plant enzyme product was evaluated through the actue toxicity test and subchronic toxicity test. The aim of actue toxicity test was in order to give the maximum tolerated dose. In the subchronic toxicity test, SD rats after 90 days continuous gavage with plant enzyme product, the general behavior, the function of liver and kidney and pathological changes of SD rats were analyzed. The result of the actue toxicity test showed that the maximum tolerated dose was up to 108mL/（kg·BW）, which was equivalent to 108 times of the recommended oral doses 60mL/（peraonal·day）. The result of the subchronic toxicity test showed that the SD rats of test group were in a good condition, and they hadn＇t the symptom of poisoning, no death and no difference in indexes of organs and histopathologic examination compared with that of blank group. The plant enzyme product had not only no toxic action in liver and kidney of rat.

关 键 词：植物酵素 安全性 急性毒性 亚慢性毒性 

分 类 号：TQ925[轻工技术与工程—发酵工程] TQ464.8