超临界CO_2萃取黔产干姜挥发油的化学成分及抑菌活性研究  

Studies on the Chemical Constituents and Antibacterial Activity of the Volatile Oil from the Rhizoma of Zingiber officinale in Guizhou province by Supercritical CO_2 Fluid Extraction

作　　者：谢晓林[1] 杨世祥[2] 胡玉松[2] 杨碧仙[1] 云雪林[3] 

机构地区：[1]贵阳学院食品与制药工程学院,贵州贵阳550005 [2]贵阳学院生物与环境工程学院,贵州贵阳550005 [3]贵阳中医学院药学院,贵州贵阳550002

出　　处：《贵阳学院学报（自然科学版）》2013年第3期15-18,共4页Journal of Guiyang University：Natural Sciences

基　　金：贵州省教育厅自然科学研究项目重点课题"萸姜药效组分方剂配伍研究"(项目编号:黔教科〔2010〕048号)阶段性成果

摘　　要：利用超临界CO2萃取法提取黔产干姜挥发油,通过气相色谱-质谱联用(GC-MS)技术对其化学成分进行分析并采用归一化法测定其相对含量;采用纸片法研究干姜挥发油对金黄色葡萄球菌和铜绿假单胞菌的抑菌活性,并用试管法测定了最低抑菌浓度。从超临界提取的黔产干姜挥发油中共分离鉴定出41个化合物,主要成分为α-姜烯(45.52%),β-倍半水芹烯(15.55%),α-法呢烯(9.79%),β-甜没药烯(6.21%),Α-姜黄烯(5.01%),姜酮(3.13%);干姜挥发油对金黄色葡萄球菌和铜绿假单胞菌均有抑制作用,经测定最低抑菌浓度均为250μL/L。Using supercritical CO2 Fluid extraction for Volatile oil from the Rhizoma Zingiberis in Guizhou province. GC - MS was used to identify the chemical compositions, and determination of the relative content of normalization method. Research antibacterial activity of the volatile oil from the Rhizoma Zingiberis by paper dish method, And the minimum inhibitory concentration was determined by the test tube method. A total of 41compounds were sparated and identified of the Volatile Oil from the Rhizoma Zingiberis by gas chromatography - mass spectrometry （ GC - MS）, major compounds in Zingiberis volatile oil were alpha - zingiberene（45.52% ）, beta - sesquiphellandrene （ 15.55% ） , alpha - farnesene （9.79%） , beta - bisabolene （ 6.21% ） , A - curcumene （ 5. O1% ） , vanillylacetone （ 3.13 % ）. The Volatile oil was found to inhibit the growth of staphylococcus aureus and pseudomonas aeruginosa. The minimum inhibitory concentration to staphylococcus aureus and pseudomonas aeruginosa were 0. 125 -0.25%.

关 键 词：黔产干姜 超临界CO2萃取挥发油 气相色谱-质谱联用技术 抑菌活性 

分 类 号：O656[理学—分析化学]