食用精炼大豆油DNA提取及单管巢式PCR检测  

DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR

作　　者：姚芹[1] 宋浩 陈枫 

机构地区：[1]苏州农业职业技术学院,江苏省食品安全快速检测工程技术研究开发中心,江苏苏州215008 [2]苏州市吴江区农业委员会,江苏苏州215200 [3]苏州市吴江区农产品检测中心,江苏苏州215200

出　　处：《食品工业科技》2013年第21期183-186,共4页Science and Technology of Food Industry

基　　金：苏州农业职业技术学院青年教师科研能力提升计划资助项目(PPN201209)

摘　　要：设计了针对转基因Roundup Ready大豆外源基因扩增的内外2对引物,分别位于CaMV35s启动子,CTP基因,CP4EPSPS基因区域,并成功对精炼大豆油中的外源基因进行单管巢式PCR检测。结果表明,用改良试剂盒法和冷冻干燥法提取大豆油中的DNA均可以满足单管巢式PCR检测要求;单管巢式PCR扩增对内外引物的Tm值有特殊要求,外引物的退火温度高于内引物。本实验建立的精炼大豆油转基因成分的单管巢式PCR检测方法特异性强,灵敏度高且快速方便。The two pairs of primers were synthesized according to genetically-modified soybean, located in the CaMV35s promoter, CTP gene, CP4EPSPS gene region, and detection method of transgenic factors by single-tube nested PCR was established.It was proved that DNA extraction by improved reagent kit method or freeze drying technology from soybean oil could meet the detection of single-tube nested PCR,and for single-tube nested PCR outer primers annealing temperature must be higher than inner primers.

关 键 词：精炼大豆油 DNA提取 CP4EPSPS基因 单管巢式PCR 

分 类 号：TS207.3[轻工技术与工程—食品科学]