新疆家蚕抗菌肽对小鼠体征指标和脏器组织影响的研究  

Effect of antimicrobial peptide CecropinXJ from Bombyx mori on physical signs indexes and organs in mice

作　　者：康苏 夏丽洁[1] 马纪[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《食品工业科技》2013年第21期343-347,共5页Science and Technology of Food Industry

基　　金：新疆维吾尔自治区高技术研究发展计划项目(201110101)

摘　　要：抗菌肽具有杀灭细菌的功能,是天然防腐剂和潜在的抗生素替代品。本研究通过对新疆家蚕抗菌肽(CecropinXJ)灌胃小鼠后相关体征指标变化和脏器组织影响的分析,检测了重组新疆家蚕抗菌肽毒性。新疆家蚕抗菌肽经大量诱导表达和分离纯化后,对小鼠进行灌胃,分析小鼠体重及脏器(肝脏、胃、脾脏和胰脏)系数的变化,观察脏器组织损伤情况。结果表明,新疆家蚕抗菌肽对小鼠体重及脏器(胃、脾脏和胰脏)系数无显著影响(p>0.05),而对小鼠的肝脏系数有影响(p<0.05)。这些结果为新疆家蚕抗菌肽抗菌产品的安全应用提供了科学依据。Antimicrobial peptide （AMP）has been considered as a potential alternative to antibiotics and can kill bacteria used as natural preservative.In this study, effect of antimicrobial peptides from Bombyx mori（CecropinXJ） on the changes of physical sign indexes in mice by gavage were observed, as well as organ damage was detected. The recombinant CecropinXJ produced by prokaryotic induced expression in large quantities was isolated and then poured into mouse stomach.The effect of CecropinXJ on the bodyweight, visceral coefficient （ liver, stomach, spleen, pancreas）in mice were analyzed and the pathological condition of visceral was observed. The results showed that CecropinXJ could not affect the body weight and visceral （ stomach, spleen, pancreas） coefficient of mice （ p 〉 0.05）, but affect liver coefficient （ p 〈 0.05）. It would provide scientific basis for safety applications of CecropinXJ products.

关 键 词：新疆家蚕抗菌肽 小鼠 体征指标 脏器系数 脏器损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]