大别山7个采样地山核桃叶的POD同工酶分析  

POD isoenzyme analysis in Carya cathayensis leaves from seven sample areas in Dabie Mountain

作　　者：薛波[1] 丁之恩[1] 李茜[1] 吕萍[1] 孙潇辉[1] 张继刚[1] 杨松[1] 

机构地区：[1]安徽农业大学茶与食品科技学院,安徽合肥230036

出　　处：《经济林研究》2013年第3期130-132,共3页Non-wood Forest Research

基　　金：中央财政林业科技成果转化与推广示范资金资助项目(〔2011〕TK047号)

摘　　要：为了提供快速鉴别大别山山核桃资源的方法，采用聚丙烯酰胺垂直平板凝胶电泳技术，对大别山地区7个采样地的山核桃叶片中的过氧化物酶（POD）同工酶酶谱特征进行了分析。结果表明：从7个采样地的山核桃叶片中共分离出了28条酶带，不同采样地的山核桃叶片同工酶酶带数各为2～6条，电泳迁移率尾值在0．310～0．470之间变化。聚类分析结果表明，当遗传距离为0．4时，可以将大别山地区的山核桃分为5类；当遗传距离为0．6时，可以分为3大类。利用过氧化物同工酶电泳图能快速而有效地区分大别山山核桃的品种。In order to provide a rapid identification method of Carya cathayensis resources, the zymogram features of POD isozyme in C. cathayensis leaves from seven different sample areas in Dabie Mountain were analyzed by using polyacrylamide vertical plating gel electrophoresis technique. The results show that 28 enzyme bands were separated, number of enzyme bands from different areas is from 2 to 6, Rf value of electrophoretic mobility is from 0.310 to 0.470. The results of cluster analysis show that they can be divided into five clusters when genetic distance is 0.4, three clusters when genetic distance is 0.6. It can be concluded that POD isoenzyme analysis is an effective method for identifying the cultivars of C. cathayensis

关 键 词：山核桃 叶片 同工酶 POD 大别山 

分 类 号：S664.1[农业科学—果树学]