4-1BBL单克隆抗体激发U937细胞NF-κB的核转位及4-1BBL/CD28同型异构分子共定位  

Anti-human 4-1BBL Monoclonal Antibody Stimulates the Nuclear Translocation of NF-κB and the Co-location of 4-1BBL/CD28 Isoform in U937 Cells

作　　者：张蔚 仇红霞 田甜 陈纬凤 王菊娟 杨向绸 许戟 段丽敏 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院血液科,老年血液科

出　　处：《中国实验血液学杂志》2013年第5期1121-1125,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金面上项目(编号81070456;81270652);六大高峰人才(编号2010-WS-019);卫生公益性行业科研专项经费项目(编号201202017);江苏高校优势学科建设工程项目(编号JX10231801)

摘　　要：本研究探讨共刺激分子4-1BBL逆向信号在人急性单核细胞白血病细胞株U937中的分子机制。选择U937细胞株作为靶细胞,与鼠抗人4-1BBL激发型单克隆抗体1F1(mAb 1F1)共培养。应用激光共聚焦显微镜观察U937细胞株上NF-κB核转位及4-1BBL分子与CD28同型异构分子(CD28i)的共定位;使用流式细胞术及RTPCR检测U937细胞4-1BBL和CD28i的分子表达。结果表明:U937细胞经mAb 1F1刺激后,出现明显的NF-κB核内转位及细胞膜上4-1BBL与CD28i分子的共定位。结论:介导U937细胞生长的4-1BBL逆向信号的传导与NF-κB的核转位相关;CD28i分子可能参与了4-1BBL逆向信号的传导。This study was purposed to investigate the molecular mechanism of 4-1BBL reverse signals in the human acute monocytic leukemia cell line of U937. The U937 cell line was used as target cells, and stimulated by the mouse anti-human 4-1BBL monoclonal antibody 1F1. The nuclear translocation of NF-KB and the co-location of 4-1BBL and CD28i molecules in U937 cells were observed with confocal laser scanning microscopy. The protein and m-RNA expres- sion levels of 4-1BBL and CD28i were detected by flow cytometry and RT-PCR respectively. The results showed that the significant nuclear translocation of NF-KB and co-localization of 4-1BBL and CD28i on membrane of U937 cells ap- peared after being stimulated by mAblF1. It is concluded that the 4-1BBL reverse signals transduction mediating the growth of U937 cells relates with the nuclear translocation of NF-KB. CD28i may be involved in intracellular 4-1BBL re- verse signaling pathways.

关 键 词：4-1BBL 逆向信号 U937细胞株 NF-ΚB CD28同型异构体 

分 类 号：R733.71[医药卫生—肿瘤]