大黄种子中蛋白质、多糖和淀粉含量的测定  

Determination of the Concentrations of Protein,Polysaccharides,Starch Detected in Rhubarb Seeds

作　　者：刘何春[1,2,3] 谭亮[1,2] 徐文华[1,2] 宋文珠[1,2] 臧黎黎 周国英[1,2] 

机构地区：[1]中国科学院西北高原生物研究所,青海省西宁市新宁路23号810008 [2]中国科学院藏药研究重点实验室,青海省西宁市新宁路23号810008 [3]中国科学院大学,北京市石景山区玉泉路19号100049 [4]电子科技大学生命科学与技术学院,成都市建设北路二段四号610041

出　　处：《光谱实验室》2013年第6期3114-3121,共8页Chinese Journal of Spectroscopy Laboratory

基　　金：国家科技支撑计划项目(2011BA105B03)

摘　　要：采用凯氏定氮法、苯酚-硫酸比色法和双波长比色法测定唐古特大黄种子、掌叶大黄种子和药用大黄种子的蛋白质、多糖和淀粉的含量。采用凯氏定氮法测定蛋白质含量,苯酚-硫酸比色法测定多糖含量,双波长比色法测定淀粉含量。唐古特大黄种子的蛋白质和多糖在三种大黄种子中含量最高,分别为21.81%、6.58%,掌叶大黄种子淀粉含量在三种大黄种子中最高,含量为32.95%。三种大黄种子中,蛋白质含量为:唐古特大黄种子>掌叶大黄种子>药用大黄种子;多糖含量为:唐古特大黄种子>掌叶大黄种子>药用大黄种子;淀粉含量为:掌叶大黄种子>唐古特大黄种子>药用大黄种子。大黄种子的蛋白质、多糖、淀粉含量丰富。测定方法精密度、准确度、稳定性均符合生物样品测定要求,可用于大黄种子蛋白质、多糖和淀粉含量的测定。The aim was to carry out a study about the concentrations of protein,polysaccharides, starch detected in the Rhubard seeds. The contents of protein were determined by kjeldahl method.The contems of polysaccharides were determined by phenol-vitriol colorimetry method. The contents of starch were determined by dual-wavelength colorimetric method. The results showed that Rheum. tanguticum seeds proteins and polysaccharides in the three authentic rhubarb seeds were 21.81%, 6.58% ,Rheum palmatum seeds starch content in the three authentic rhubarb seeds were 32.95M. Protein content of three authenIic rhubarb seeds were：Rheum, tanguticum seeds Rheum palmatum seeds ） Rh. officinale seeds; polysaccharide content were： Rheum. tanguticum seeds ）Rheum palmatum seeds 〉 Rh. officinale seeds; starch content were. Rheum palmatum seeds 〉 Rheum tanguticum seeds〉 Rh. officinale seeds. Rhubarb seeds contain abundant protein, polysaccharides; starch. The methods is proved to be accurate, precise, stable and suitable for the protein, polysaccharides and starch in Rhubarb seeds.

关 键 词：大黄种子 蛋白质 多糖 淀粉 

分 类 号：O655.2[理学—分析化学] O657.32[理学—化学]