食管癌及癌前病变组织中癌基因的表达  

STUDIES ON ONCOGENE IN THE ESOPHAGUS CARCINOMA AND PRECANCEROSIS

作　　者：张式暖[1] 王亚利[1] 王艳华[1] 吕世军[1] 魏志新[1] 姜红心[1] 

机构地区：[1]潍坊医学院病理学教研室,261042

出　　处：《中国现代医学杂志》2000年第9期8-9,共2页China Journal of Modern Medicine

基　　金：山东省教委资助!项目 编号 19996

摘　　要：目的 :研究 p5 3基因点突变在食管癌及癌前病变粘膜细胞癌变过程中的变异作用及定量分析 ,对病人预后的关系。方法 :选取手术新鲜标本及蜡块 90例。DNA提取分析 ,应用PCR -RFLP和DNA序列分析 ,免疫组化ABC法 ,利用MPV - 2型显微分光光度计检测分析。结果 p5 3基因在食管癌、不典型增生组织中点突变频率较高 ,分别为 6 0 %、2 9.4%。错义突变和移码突变占全部突变的 73%。p5 3表达强度与生存期有相关性。结论 :p5 3基因点突变在食管粘膜细胞癌变过程中起重要作用。Objective: To investigate the variation action of p53 point mutation in esophagus carcinoma, precancerosis and the p53 quantitative analysis relationship with patient's prognosis. Methods: Selected 90 cases of fresh operative preparations and embedded (precancerosis 30, squamous cell carcinoma 60). DNA advance analysis, used PCR-RFLP and DNA sequential analysis to examine and analyze the fashions and types of p53 gene point mutation; By Immunohistochemistry ABC methods. Results: Frequencies of p53 gene point mutation in esophagus carcinoma and dysplasia tissues were higher (60% and 29.4% respectively). Missense mutation and frame shift mutation accounted for 73 percent of all. Expression of p53 positive immunoactive cells was 60% in esophagus carcinoma and 42% in dysplasia. The degree of p53 gene expressions had a relationship with survival stage. Compared esophagus carcinoma and dysplasia with normal mucosa tissue, the activity of LDH increased P <0.05. Compared the numbers of monocyte and lymphocyte of stroma of esophagus carcinoma with that of normal mucosa cell had difference P <0.01. Conclusions: p53 gene point mutation plays an important role in the processes of esophagus carcinomas.

关 键 词：食管肿瘤 癌基因蛋白p53 点突变 癌前状态 病理 

分 类 号：R735.102[医药卫生—肿瘤]