菌株ZD8的分离鉴定及其异养硝化和缺氧/好氧反硝化特性研究  

Isolation and identification of a bacterial strain ZD8 and its characteristics of heterotrophic nitrification and anoxic /oxic denitrification

作　　者：赵丹[1] 于德爽[1] 李津[1] 汪晓晨[1] 

机构地区：[1]青岛大学化学化工与环境学院,青岛266071

出　　处：《环境科学学报》2013年第11期3007-3016,共10页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助项目(No.51078191;51278258);青岛市应用基础研究项目(No.13-1-4-203-jch)~~

摘　　要：从稳定运行的ASBR厌氧氨氧化反应器中分离筛选出一株在缺氧和好氧条件下均具有高效反硝化能力的菌株ZD8,该菌株为假单胞属（Pseudomonas sp.）,大小2 μm0.25 μm,无鞭毛和芽孢.实验结果表明,缺氧条件下,ZD8最适合的碳源为柠檬酸钠;当C/N为10时,具有最佳的反硝化效果.菌株ZD8在缺氧条件下不具有硝化能力.在好氧条件下菌株ZD8获得最佳反硝化效果的C/N为22,最适合pH范围是7.2~9.9.菌株ZD8在好氧条件下具有高效的异养硝化能力,NH4＋-N平均去除速率为8.3 mgL-1h-1.当以KNO3为氮源时ZD8的反硝化速率为13.1 mgL-1h-1;而以NaNO2为氮源时,其反硝化速率为6.98 mgL-1h-1.在同时存在NH4＋-N和NO3--N或NH4＋-N和NO2--N的系统中,菌株ZD8均首先利用NH4＋-N发生硝化作用,NH4＋-N的存在对反硝化具有抑制作用,并且NH4＋-N对NO2--N的反硝化抑制作用更强;在同时存在NO3--N和NO2--N的系统中,菌株ZD8优先利用NO3--N进行好氧反硝化脱氮.A bacterial strain named ZD8 was isolated and identified from an ASBR anaerobic ammonia oxidation reactor. Its size was 2 μm0.25 μm without flagellum and spore. The experimental results showed that under anoxic condition, the optimal carbon resource for ZD8 was sodium citrate;when C/N was 10, the strain had optimal denitrification efficiency. Under anoxic condition, ZD8 did not have heterotrophic nitrifying ability. However, under oxic condition, the optimal denitrification efficiency appeared when C/N was 22 and the optimal pH range was 7.2~9.9. Under anoxic condition, strain ZD8 had good heterotrophic nitrification capacity, and the average removal rate of NH4＋-N was 8.3 mgL-1h-1. When KNO3 was utilized as nitrogen source, the average removal rate of NO3--N was 13.1 mgL-1h-1. When NaNO2 was utilized as nitrogen source, its removal rate was 6.98 mgL-1h-1, In the mixed media of ammonium-nitrate or ammonium-nitrite, ZD8 was preferred to use ammonium to perform heterotrophic nitrification;the ammonium had inhibition on denitrification, and the inhibition to nitrite denitrification was more serious than nitrate denitrification. The strain preferred nitrate as the N source in the nitrate-nitrite mixed media.

关 键 词：缺氧 好氧反硝化 异养硝化 脱氮 去除速率 系统发育分析 

分 类 号：X172[环境科学与工程—环境科学] X703.1