双水相萃取-流动注射化学发光法测定果蔬中残留α-萘乙酸  

Determination of α-Naphthaleneacetic Acid in Vegetables and Fruits by Flow Injection Chemiluminescence with Aqueous Two-Phase Extraction

作　　者：高向阳[1,2] 王珊[2] 陈双[1] 朱盈蕊[3] 

机构地区：[1]郑州科技学院食品科学与工程学院,河南郑州450064 [2]河南农业大学食品科学技术学院,河南郑州450002 [3]郑州市质量技术监督检验测试中心,河南郑州450006

出　　处：《食品科学》2013年第20期213-216,共4页Food Science

基　　金：河南省重点学科建设基金资助项目(10466-X-082301)

摘　　要：建立一种快速测定果蔬中残留α-萘乙酸的新方法。α-萘乙酸经乙醇-(NH4)2SO4溶液双水相体系萃取,利用其对Luminol-H2O2-Cr3+化学发光体系的抑制作用进行测定。结果表明:方法的线性范围为1.0×10-9～1.0×10-4mol/L,r=0.9976,对1.0×10-8mol/Lα-萘乙酸进行11次平行测定的相对标准偏差为3.2%,按3倍标准偏差计算的方法检出限为3.98ng/g,平均加标回收率为88.9%～92.1%。该法线性范围宽、灵敏度高、易实现自动化,用于测定果蔬中残留的α-萘乙酸,结果令人满意。A flow injection chemiluminescence method was established for the fast determination of α-naphthaleneacetic acid （NAA） in vegetables and fruits based on its suppressive effect on the reaction between Luminol and hydrogen peroxide. NAA residue was extracted with ethanol/ammonium sulfate. The linear range of this method was 1.0 × 10-9 to 1.0 × 10-4 mol/L, with correlation coefficient of 0.9976, the detection limit was 3.98 ng/g, the relative standard deviation （RSD） was 3.2% for 11 parallel determinations, and the recovery rate was 88.99% to 92.1%. This method had the advantages of wide linear range, high sensitivity and ease of automatic operation and was applied with satisfactory results in the determination of NAA in vegetables and fruits.

关 键 词：双水相萃取 流动注射化学发光 果蔬 α -萘乙酸 

分 类 号：O657.39[理学—分析化学]