绳状青霉β-葡萄糖苷酶的分离纯化及其酶学性质研究  

Study on Purification and Characterization of A β-Glucosidase from Penicillium Funiculosumand Its Enzymatic Properties

作　　者：张旭[1] 刘华晶[1] 黄晓梅[1] 李倩[1] 徐诚蛟[1] 

机构地区：[1]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《化学与生物工程》2013年第10期38-41,共4页Chemistry & Bioengineering

基　　金：东北农业大学大学生创新团队发展计划项目(CXT 003-2-1)

摘　　要：从绳状青霉(Penicillium funiculosum)深层发酵液中分离得到一种未知的β-葡萄糖苷酶,并对其酶学性质进行初步分析。粗酶液经硫酸铵沉淀、DEAE-Sephadex A-25离子交换层析、Sephadex G-50凝胶过滤层析等步骤,获得了一种凝胶电泳均一的β-葡萄糖苷酶,经SDS-PAGE测得其分子量为19kDa,其最适反应温度为60℃、最适反应pH值为4.5。A β-glucosidase was purified and prelimarily characterized from submerged fermentation of Peni- cilliurnfuniculosum. The β-glucosidase was purified by ammonium sulfate precipitation, DEAE-Sephadex A-25 anion-exchange chromatography and Sephadex G-50 gel-filtration chromatography. SDS-PAGE Showed the pu- rified enzyme to be a homogeneous protein with molecular weight of 19 kDa. Its optimal reaction temperature and pH value were 60 ℃ and 4.5,respectively.

关 键 词：绳状青霉 Β-葡萄糖苷酶 纯化 酶学性质 

分 类 号：Q814[生物学—生物工程] S216.3[农业科学—农业机械化工程]