人乳腺球蛋白、血管内皮生长因子-C和血管内皮生长因子受体-3在乳腺癌中的表达及其与预后的关系  

Expression of hMAM,VEGF-C and VEGFR-3in breast cancer tissues and its relation with prognosis

作　　者：刘新兰[1] 黄英[1] 陈萍[1] 

机构地区：[1]宁夏医科大学总医院肿瘤内科,银川750004

出　　处：《第二军医大学学报》2013年第10期1078-1082,共5页Academic Journal of Second Military Medical University

基　　金：教育部春晖计划合作科研项目(Z2008-1-75004)~~

摘　　要：目的探讨人乳腺球蛋白(hMAM)、血管内皮生长因子-C(VEGF-C)及其受体-3(VEGFR-3)在乳腺癌组织中表达,分析其表达与乳腺癌生物学特性及预后的关系。方法采用组织微阵列技术,通过免疫组化法检测116例乳腺癌组织、44例癌旁正常组织中hMAM、VEGF-C及VEGFR-3的表达。结果 (1)乳腺癌组织中hMAM、VEGF-C及VEGFR-3的表达率分别为59.48%(69/116),50.86%(59/116)及61.20%(71/116),均高于癌旁正常组织中的表达率(分别为0.00%、9.09%及18.18%,P均<0.01)。(2)hMAM、VEGF-C及VEGFR-3的表达与腋淋巴结转移呈正相关(P均<0.05),hMAM和VEGFR-3的表达与组织学分级呈正相关(P均<0.05)。(3)采用Spearman等级相关分析结果显示,hMAM、VEGF-C及VEGFR-3在乳腺癌中的表达两两之间均呈正相关,相关系数分别为0.278、0.280及0.244(P均<0.01)。(4)Kaplan-Meier生存分析结果表明:hMAM、VEGF-C及VEGFR-3阴性表达者无病生存率及5年总生存率均高于三者阳性表达(Log-rank,P均<0.01)。结论 hMAM、VEGF-C及VEGFR-3在乳腺癌组织中存在高表达,其高表达与乳腺癌的发生、侵袭转移和预后有关,且hMAM可能与乳腺癌微淋巴管生成有关。Objective To study the expression of human-mammaglobin （hMAM） and vascular endothelial growth factor C （VEGF-C） and vascular endothelial growth factor receptor 3 （VEGFR-3） in breast cancer tissues, and to analyze its relationship with biological characteristics and prognosis of breast cancer. Methods The expression of hMAM, VEGF-C andVEGFR-3 was determined by immunohistochemical technique in 116 breast cancer tissues and 44 adjacent normal tissues （from cancer tissue≥5 cm） using tissue microarray technology （TMA）. Results （1） The positive rates of hMAM, VEGF-C and VEGFR-3 in primary breast carcinoma tissues were significantly higher than those in the adjacent normal tissues （ 59.48% ／[69/116／] vs 0.00%, 50.86% ／[59/116／] vs 9.09% and 61.20% ／[71/116／] vs 18.18%, respectively, all P〈0.01）. （2）The positive rates of hMAM , VEGF-C and VEGFR-3 were significantly correlated with axillary lymph node metastasis, and the positive rates of hMAM , VEGFR-3 were significantly correlated with histological gradings （all P〈0.05）. （3） Spearman rank correlation analysis showed that the positive rates of hMAM, VEGF-C and VEGFR-3 in breast cancer tissues were all significantly correlated with each other, with correlation coefficients being 0.278, 0.280, and 0.244, respectively, all P〈0.05）. （4）Kaplan-Meier survival analysis showed that the disease-free survival and 5-year overall survival of patients with negative expression of HMAM, VEGF-C and VEGFR-3 were significantly prolonger compared to those with positive expression （Log-rank test, P〈0.05）. Conclusion hMAM, VEGF-C and VEGFR-3 are highly expressed in breast cancer patients, which might be associated with the invasion and metastasis of breast cancer, and hMAM expression might be related to the lymphangiogenesis of breast cancer.

关 键 词：乳腺肿瘤 人乳腺球蛋白 血管内皮生长因子C 血管内皮生长因子受体3 微阵列分析 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]