K-ras基因突变两种检测方法的比较  

Comparison between two methods of K-ras mutations detection

作　　者：许洁[1] 李丽[1] 张科平[1] 骆新兰[1] 庄恒国[1] 刘艳辉[1] 

机构地区：[1]广东省人民医院广东省医学科学院病理科,广州510010

出　　处：《诊断病理学杂志》2013年第10期629-633,共5页Chinese Journal of Diagnostic Pathology

摘　　要：目的通过比较ARMS实时荧光定量PCR法和DNA直接测序法,分析K-ras基因突变检测结果的一致性,确立临床K-ras基因突变检测的最适方法。方法应用ARMS实时荧光定量PCR法及DNA直接测序法检测34例石蜡标本的K-ras基因第12位和第13位密码子的基因突变,结果比较采用χ2检验。结果这两种方法对K-ras基因第12和第13密码的基因突变检测结果无统计学差异,ARMS实时荧光定量PCR法比测序法操作简便,敏感度更高。结论相较于DNA直接测序法,ARMS实时荧光定量PCR法更适用于临床检测。Objective To compare the two methods of ARMS real-time PCR and DNA sequencing in detection of K-ras mutations. Methods K-ras 12th and 13th codon gene mutation were detected by ARMS real-time PCR and DNA sequencing in 34 paraffin specimens. Chi-square test was used to analyze the consistance. Results There was no significant difference between the two methods. However, ARMS real-time PCR was more convenient and sensitive compared to DNA sequencing. Conclusion ARMS real-time PCR is more suitable for clinical testing than DNA sequencing.

关 键 词：K-RAS基因 基因突变 ARMS 荧光定量PCR DNA直接测序法 

分 类 号：R737.9[医药卫生—肿瘤]