轻,中、重度慢性牙周炎多种等位基因的检测  

Detection of Several Alleles in Mild Periodontitis and Moderate to Severe Periodontitis

作　　者：王宏岩[1] 谭丽思[1] 赵海礁[1] 李倩[1] 潘亚萍[1] 

机构地区：[1]中国医科大学附属口腔医院牙周科,辽宁沈阳110002

出　　处：《口腔医学研究》2013年第10期907-910,共4页Journal of Oral Science Research

基　　金："十一五"国家科技攻关计划(编号:2007BAI18B02)

摘　　要：目的:探讨轻度慢性牙周炎个体和中、重度慢性牙周炎个体携带不同数目牙周炎易感基因的差异。方法:对纳入的慢性牙周炎个体和健康对照组进行牙周临床检查,并抽取外周静脉血,提取基因组DNA,应用限制性内切酶,采用聚合酶链反应限制性片段长度多态性的方法检测HLA-DRB1*1501、TNF-A-308、IL-1B+3953、雌激素受体、维生素D受体5种基因的多态性。结果:中、重度慢性牙周炎个体携带3种以上易感等位基因的百分比为56.6%,轻度慢性牙周炎个体携带3种以上易感等位基因的百分比为12.5%,健康对照组携带3种以上易感等位基因的个体百分比为7.5%,经方差分析中、重度慢性牙周炎组与轻度慢性牙周炎组和健康对照组间差异具有统计学意义(P<0.01),轻度慢性牙周炎组与健康对照组间差异不具有统计学意义。中、重度慢性牙周炎组携带VDR-TT、ER-XX基因型的个体临床附着丧失重于携带VDR-tt和ER-xx基因型的个体,经t检验二组差异具有显著性(P<0.01)。结论:多种等位基因的共同携带有助于慢性牙周炎的进展;VDR-TT和ER-XX基因型可能是牙周骨组织丧失的危险因素。Objective： To investigate the difference between mild chronic periodontitis and moderate to severe chronic periodontitis on carrying susceptible alleles. Methods： We examined the periodontal clinical indexs in included individuals. Genomic DNA was extracted from peripheral venous blood. Polymerase chain reaction and cleavage were used for detecting the frequencies of five susceptible gene polymorphism in chronic periodontitis patients and healthy controls. Results： The percentage of the moderate to severe chronic periodontitis patients who taking more than three susceptible alleles was 56.6 %, in mild chronic periodontitis was 12.5 %,in healthy controls was 7.5 %. The difference among the moderate to severe chronic periodontitis patients, mild chronic periodontitis and healthy con- trois was statistically significant（P^0.01）, but no difference was found between the mild periodontitis patients and controls. The clinical attachment loss in moderate to severe chronic periodontitis patients who taking VDR-TT, ER-XX alleles was more than which in moderate to severe chronic periodontitis patients who taking VDR-tt, ER -xx alleles . The difference between the two groups was statistically significant. Conclusion： Carrying more than three susceptible alleles has important effect on the development of chronic periodontitis. VDR-TT and ER-XX genotype may be susceptible indicator for chronic periodontitis.

关 键 词：慢性牙周炎 等位基因 牙周临床检查 频率 

分 类 号：R781.4[医药卫生—口腔医学]