PARP及NF—κB在重症急性胰腺炎大鼠肾上腺细胞中的表达及5-AIQ／PDTC的干预作用  

Expression of PARP/NF-κB and intervention effect of 5-AIQ/PDTC in SAP rats with adrenal damage

作　　者：杨波[1] 郭闻一[2] 余佳[2] 赵凯亮[2] 石乔[2] 左腾[2] 王卫星[2] 

机构地区：[1]首都医科大学附属北京安贞医院心外一科,100029 [2]武汉大学人民医院肝胆外科

出　　处：《中华医学杂志》2013年第38期3063-3067,共5页National Medical Journal of China

基　　金：国家自然科学基金（81070368）;湖北省卫生厅科研重点项目（ZX6A07）

摘　　要：目的探讨ADP核糖聚合酶（PARP）／核因子（NF）-κB在大鼠重症急性胰腺炎（SAP）肾上腺损伤中的表达情况及PARP抑制剂5-氨基异喹啉酮（5-AIQ）／吡咯烷二硫代氨基甲酸盐（PDTC）的干预作用。方法将原代培养的肾上腺皮质细胞进行细胞定量后分为6组：空白对照（SO组）、胰腺炎组（SAP组）、PDTC药物对照组（SO＋PDTC组）、PDTC干预组（SAP＋PDTC组）、5-AIQ药物对照组（SO＋5-AIQ组）和5-AIQ干预组（SAP＋5-AIQ组）。以胰腺炎大鼠血清刺激SAP组和2个干预组，之后以最适浓度加入每组相应药物，在给药后继续培养12h，观察各组细胞皮质酮分泌情况及PARP／NF-κB的表达情况。结果体外培养肾上腺细胞呈圆形或类圆形，胞质内有分泌颗粒，培养第2天贴壁率约60％，可被3β-HSD染色。SAP组皮质酮水平为（216．4±15．7）μg/L，明显低于SO组（294．8±16．3）μg/L（P〈0．05），5-AIQ干预组和PDTC干预组皮质酮水平分别为（258．6±19．0）μg／L及（264．3±18．2）μg／L，明显高于SAP组但低于SO组和药物对照组（P〈0．05）；SAP组和PDTC干预组肾上腺细胞PARP-1的表达高于SO组，5-AIQ干预组PARP-1的表达明显低于SAP组和PDTC干预组，但高于SO组和药物对照组；SAP组肾上腺细胞NF-κB的表达高于SO组，5-AIQ干预组和PDTC干预组NF-κB的表达明显低于SAP组，但高于SO组和药物对照组。结论PARP／NK-κB通路参与重症急性胰腺炎肾上腺损伤过程，应用5-AIQ及PDTC可在一定程度上减轻肾上腺细胞功能的损伤程度。Objective To explore the expression of poly （ADP-ribose） polymerase/nuclear factor- κB （PARP/NF-κB） and intervention effect of 5-aminoisoquinolinone/pyrrolidine dithiocarbamate （5-AIQ/ PDTC） in severe acute pancreatitis （SAP） rats with adrenal damage. Methods The primarily cultured adrenocortical cells were quantitatively divided into control group （SO）, panereatitis group （SAP）, PDTC drug control group （ SO ＋ PDTC ） , PDTC intervention group （ SAP ＋ PDTC ）, 5-AIQ drug control group （ SO ＋ 5-AIQ） and 5-AIQ intervention group （SAP ＋ 5-AIQ）. The SAP and 2 intervention groups were stimulated with the sera of SAP rats. Then colresponding drugs were added and culture continued for 12 hours. The corticosterone levels and PARP/NF-κB expression were observed for each group. Results Adrenal ceils in vitro cultured were round or oval, had secretory granules and could be stained by 3β- hydroxysteroid dehydrogenase antibody. The adherence rate was 60% after 48-hour culturing. The corticosterone level of SAP group was significantly lower than that of SO group [ （ 216.4± 15.7 ） vs （294. 8 ± 16. 3 ） μg/L, P 〈 0. 05 ]. The 2 intervention groups were （ 258. 6 ± 19.0 ） and （ 264. 3± 18.2 ） μg/L respectively. These two values were higher than those of SAP group （ P 〈 0. 05 ）, but lower than thoseof SO group （P 〈 0.05）. With regards to the expression of PARP-1, the SAP and PDTC intervention groups were higher than SO group while 5-AIQ intervention group was significantly lower than SAP and PDTC intervention groups, but higher than SO and drug control groups. The expression of NF-κB in SAP group was higher than that in SO group. Two intervention groups were lower than SAP group, but higher than SO and drug control groups. Conclusion The pathway of PARP/NF-κB participates in adrenal damage of SAP rats. To a certain extent, the uses of 5-AIQ and PDTC may alleviate adrenal damage.

关 键 词：肾上腺皮质细胞 重症急性胰腺炎 PARP NF-κB 抑制剂 

分 类 号：R576[医药卫生—消化系统]