番茄红素在鱼藤酮诱导的PC12细胞凋亡中的作用  

The effect of lycopene on rotenone-induced apoptosis in PC12 cells

作　　者：罗连响[1] 李晓玲[2] 鲍波[1,3] 

机构地区：[1]广东医学院病理生理教研室,广东湛江524023 [2]广东医学院微生物学与免疫学教研室,广东湛江524023 [3]广东医学院实验动物学教研室,广东湛江524023

出　　处：《中风与神经疾病杂志》2013年第10期884-886,共3页Journal of Apoplexy and Nervous Diseases

基　　金：广东省科技计划项目(2010B060500015)

摘　　要：目的探讨番茄红素对鱼藤酮诱导的PC12细胞凋亡的作用及可能机制。方法采用鱼藤酮诱导PC12细胞为损伤模型,在番茄红素预处理后,以CCK-8法检测细胞存活率,Hoechst33258染色荧光显微镜观察细胞凋亡形态学改变,流式细胞术检测细胞凋亡率,罗丹明123染色检测细胞线粒体膜电位,比较各组的差异。结果与鱼藤酮组相比,番茄红素预处理后,细胞存活率明显提高(P<0.05),细胞形态明显改善,凋亡率降低(P<0.05),线粒体膜电位上升(P<0.05)。结论番茄红素对鱼藤酮诱导的PC12细胞凋亡具有抑制作用,其机制可能与其稳定线粒体膜电位有关。Objective To investigate lycopene on rotenone induced apoptosis in PC12 cells and its possible mecha- nism. Methods Rotenone-induced PC12 cells were used in the study. Cell viability was determined by CCK-8 assay,The morphological change of PC12 cells was observed by Hoechst33258 staining, The apoptosis was monitored by FCM, Mito- chondrial membrane potential（MMP） of PC12 cells was detected by Rh123 staining. Results Lycopene significantly atten- uated the cytotoxic effect of rotenone. It increased the cell survival as measured with CCK-8, decreased the apoptosis rate, and inhibited MMP collapse. Conclusion Lycopene has a possible neuroprotective role in rotenone-induced apoptosis of PC12 cells, and the mechanism of the effects of lycopene may be involved in inhibiting MMP collapse induced by rotenone.

关 键 词：番茄红素 鱼藤酮 PC12 细胞凋亡 

分 类 号：R742[医药卫生—神经病学与精神病学]