D-半乳糖诱导建立的小鼠多囊卵巢综合征模型  

A mouse model of polycystic ovary syndrome induced by D-galactose

作　　者：周越[1] 董英[1] 陆嘉伟[1] 何远清[2] 费倩[1] 蔣王英 李思斌[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学实验动物中心,江苏镇江212013

出　　处：《中国实验动物学报》2013年第5期19-24,I0006,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家高技术研究发展(863)计划项目(No.2011AA100803);江苏省优势学科建设工程资助项目;江苏省高等学校大学生实践创新训练计划项目(No.2012JSSPITP1281)

摘　　要：目的研究D-半乳糖诱导ICR中年雌性小鼠多囊卵巢综合征(PCOS)动物模型的卵巢形态学、性激素以及胰岛素水平变化,并探讨D-半乳糖引致小鼠PCOS的意义。方法以D-半乳糖腹腔注射20周龄ICR雌性小鼠8周,观察卵巢形态的变化,检测血糖值及动情周期排卵情况,并采用ELISA法测定血清胰岛素、雌二醇(E2)、促卵泡生长激素(FSH)、睾酮(T)水平。结果 D-半乳糖处理组小鼠的卵巢重量显著高于对照组(P<0.05),有80%(10/12)的单侧或双侧卵巢呈现多囊性扩张,卵巢闭锁增多及颗粒细胞层数减少,并表现为紊乱的动情周期,提示无排卵;与对照组比较,D-半乳糖组小鼠血清T、E2和空腹血糖水平明显升高(P<0.001),FSH水平下降(P<0.0001),空腹血胰岛素水平显著高于对照组(P<0.01),胰岛素敏感指数显著低于对照组(P<0.05)。结论使用D-半乳糖诱导小鼠PCOS模型,无论在影响血清性激素还是卵巢局部形态学改变方面都与临床表现相似,并存在胰岛素抵抗现象,符合PCOS的典型特征,可作为动物模型用于科学研究。Objective To investigate the morphological changes in the ovaries and the serum levels of several related hormones, insulin and glucose in the mouse model of polycystie ovary syndrome （PCOS） induced by D-galactose, and to discuss their significance. Methods 20-week-old female ICR mice were injected daily （ i. p） with D-galactose for 8 weeks. ELISA was used to determine the serum levels of follicle-stimulating hormone （FSH） , estrogen （E2） , testosterone （T） and insulin （INS）. HE staining was used for the pathological examination of morphological changes of the ovaries. The stages of estrous cycle were verified by vaginal cytology. Results The ovaries of the D-galactose-treated mice showed polycystie changes of ovarian cysts and atretic follicles. Abnormal estrous cycles were induced in the D-galaetose-treated mice. Comparing with the normal group, the serum estrogen （E2 ） and testosterone （T） were significantly increased and serum FSH was reduced in the D-galactose group （P 〈 0. 001 or P 〈 0. 0001 ）. The level of serum insulin was significantly higher in the D-galactose-treated mice than in the normal group （ P 〈 0. 01 ）. In comparison with the normal group, the val- ue of 1/（ INS × G） was significantly decreased in the D-galactose-treated mice （P 〈 0.05）. Conclusions The level of serum sex hormones and the ovarian morphology of the D-galactose-treated mice are similar to those of the patients with P- COS, and insulin resistance is observed. Our results indicate that the D-galactose-treated mouse model may be a useful animal model for future experimental research of polycystic ovary syndrome.

关 键 词：D-半乳糖 PCOS模型 激素 卵巢形态 动情周期 小鼠 

分 类 号：Q95-33[生物学—动物学]