肿瘤体外三磷酸腺苷药敏试验  

The Evaluation of Adenosine Triphosphate Bioluminescence Assay for Tumor in Vitro Chemosensitivity Test

作　　者：刘珊玲[1] 彭芝兰[1] 楼江燕 王和[1] 唐茜萍[1] 何斌[1] 

机构地区：[1]华西医科大学附二院妇产科教研室,成都610041

出　　处：《肿瘤防治研究》2000年第6期429-432,共4页Cancer Research on Prevention and Treatment

基　　金：高校博士点基金!课题资助项目(卫科技发(91)第 10 1号 ;校内编号 910 14)

摘　　要：目的　评价肿瘤细胞经药物处理后的生存情况 ,探讨将该方法用于卵巢癌药敏试验的可行性。方法　采用生物荧光法检测三磷酸腺苷 (ATP) ,对小鼠纤维瘤细胞株L92 9、人卵巢癌组织和腹水细胞进行抗癌药物处理后的细胞活性测定。结果　该法能测出不同浓度药物对细胞作用的剂量—效应关系 ,以及不同个体对不同浓度药物反应的差异 ,实验变异系数CV值为 1 2～ 15 8%。结论　ATP生物荧光法有稳定、重复性好、特别敏感的特点 ,用于卵巢癌体外药敏试验可行。Adanosine triphosphate(ATP) is the basic energy source for living cells.It rapidly disappears when cells die.So measuring the ATP levels may estimate the viability of cells exposed to drugs.Dose-response curve can be determined in mouse fibroma cell line:L929 treated with chemotherapeutic agents by the ATP-bioluminescence assay and The different in vitro response of 6 ovarian carcinoma (5 from fresh tumor tissues,1 from ascites)treated with etopside,cis-plating,5-fluorouracil and adriamycin can be investigated.The coefficient of variation for triplicate assays ranges from 1.2 to 15.8%.It means high reproducibility of the assay.This presentation also reported that single cell suspension(include <30 cells clusters) can be separated from tissue fragments by means of the enzyme cocktail(collagenase,Dnase,pronase).The viable cells were over 90%.0.5% Argarose/McCoy underlayer can prevent the growth of anchorage-dependent nonmalignant cells.This study demonstrates that ATP bioluminescence assay is a sensitive,efficient method for tumor chemosensitivity test.Correlation of in vitro drug sensitivity and in vivo patient response worth further studying.

关 键 词：卵巢癌 三磷酸腺苷 生物荧光法 体外药敏试验 

分 类 号：R737.310.5[医药卫生—肿瘤] R73－36[医药卫生—临床医学]