透明质酸发酵过程控制的研究  

Research on Control of Fermentation of NW-162 Expressing HA

作　　者：赵亚玲[1] 惠有为[1] 谢景山[1] 

机构地区：[1]西北大学化工学院,陕西西安710069

出　　处：《广东化工》2013年第19期1-2,共2页Guangdong Chemical Industry

基　　金：陕西省教育厅科研基金资助项目(01JK77)

摘　　要：进行了兽疫链球菌NW-162高密度发酵HA的工艺研究,通过优化了发酵过程中不同饱和度的溶解氧和不同PH调控方法对该菌株发酵生产透明质酸的影响。试验表明:采用30%饱和度的溶解氧和在发酵过程的0～30小时,调pH 7.2,发酵后期调pH 6.8的调控方法,OD660可达0.84,透明质酸产量为0.532 g/L。在此条件下,有利于细胞生长和产物表达,降低乙酸等有害代谢副产物积累。The high cell-density fenmentation process for producing hyaluronic acid with a Streptococcus zooepidemics mutant NW-162 was studied. The different dissolved oxygen （DO） and differeut pH was optimized during fermentation process. The result showed the maximal yield of hyaluronic acid was obtained to 0.532 g.L^-1 and DO660 value reached 0.84, when the dissolved oxygen was controlled at 30 % and pH value was at pre-term and mid-term pH 7.2, final-term pH 6.8 during different terms of fermentation. Under the conditions, it was beneficial to the growth of cell and aim product expression and it could reduce the accumulation of acetic acid.

关 键 词：兽疫链球菌 高密度发酵 透明质酸 

分 类 号：Q815[生物学—生物工程]