嗜酸乳杆菌高密度培养工艺条件的优化及其评价  

Optimization and evaluation of process condition of high density culture of Lactobacillus acidophilus

作　　者：宋艳[1] 李岳飞[1] 王智鼎[1] 宋帅[2] 孙德军[1] 

机构地区：[1]吉林大学再生医学科学研究所生物技术药物教研室,吉林长春130021 [2]吉林农业大学发展学院生物制药技术教研室,吉林长春130600

出　　处：《吉林大学学报（医学版）》2013年第5期1036-1040,共5页Journal of Jilin University：Medicine Edition

基　　金：国家高技术研究发展计划(863)项目资助课题(2006AA020503)

摘　　要：目的:探讨嗜酸乳杆菌培养条件,研制出一种菌活性高、密度大、操作简便、易于生产应用的发酵工艺,阐明乳酸菌直投式发酵剂的生产和应用。方法:选取嗜酸乳杆菌中国株为菌种,根据培养基成分的配比不同,分为实验组和对照组,优化培养条件以及MRS液体培养基的配方,通过紫外分光光度计检测菌液的吸光度(A650)值和活菌数量变化,筛选出最优的工艺过程。结果:通过筛选确定嗜酸乳杆菌的最佳培养条件是接种量1%,初始pH值6.2,37℃恒温厌氧培养23h;最适培养基配方为3.5%蛋白胨、0.5%酵母提取物、2%葡萄糖、0.2%柠檬酸铵、0.088%磷酸氢二钠、0.6%乙酸钠、0.025%硫酸锰、0.06%硫酸镁、1%营养因子和0.1%吐温80;由最适培养工艺培养得到的菌种数可达1.43×1011 cfu·mL-1,较传统培养方案相比菌种数增长约1×1011 cfu·mL-1,且工艺简便易行。结论:实现对嗜酸乳杆菌培养条件和培养基配方的优化,得到嗜酸乳杆菌最优培养工艺条件,菌种的高密度培养可用于乳酸菌直投式发酵剂的制备和应用。Objective To study the culture conditions of Lactobacillus acidophilus,and to develop a fermentation with high bacterial activity,big density,simple operation,easy for production application,and to clarify the production and application of lactic acid bacteria.Methods Lactobacillus acidophilus strain of Chinese strains were selected;according to the proportion of different medium components,experimental group and control group were set up;the culture conditions and formula of MRS liquid culture medium were optimized,and the A650 value of bacteria liquid and the number of live bacteria were detected by UV spectrophotometer,and the optimal process was screened out.Results Through screening the optimum culture conditions of Lactobacillus acidophilus were determined;the inoculation quantity was 1%,initial pH value was 6.2,the constant temperature was 37 ℃ and the culture time was 23h..The optimum culture medium formula was peptone 3.5%,yeast extract 0.5%,2% glucose,0.2%citric acid ammonium,0.088% sodium hydrogen phosphate two,0.6% sodium acetate,0.025% manganese sulfate,magnesium sulfate 0.06%,1% nutrition factor,0.1% Twain 80;the number of bacteria was up to 1.43× 1011 cfu·mL-1 cultured with the optimal culture process;compared with the traditional training scheme,the number of bacteria species was increased about 1×1011 cfu·mL-1,and the process was simple and easy.Conclusion Through the optimization of culture conditions and culture medium of Lactobacillus acidophilus,the best culture conditions of Lactobacillus acidophilus are obtained,the high density cultivation of strain is conduted,and a theoretical foundation for preparation and application of lactic acid bacteria is laid.

关 键 词：嗜酸乳杆菌 高密度 培养条件 优化 工艺 

分 类 号：TS201.3[轻工技术与工程—食品科学]