乳腺浸润性导管癌染色体1p36杂合性缺失的初步研究  

Primary study of loss of heterozygosity on chromosome 1p36 in invasive ductal breast carcinoma

作　　者：林瀛[1] 陈小岩[1] 何毅辉[1] 晋龙[1] 俞训彬[1] 

机构地区：[1]福建医科大学省立临床医学院福建省立医院病理科,福州350001

出　　处：《肿瘤研究与临床》2013年第10期654-658,共5页Cancer Research and Clinic

基　　金：福建省卫生厅青年科研基金（2010-0204）

摘　　要：目的 探讨染色体1p36可能存在的与非特殊类型乳腺浸润性导管癌发生、发展有关的肿瘤抑制基因,为发现和定位肿瘤抑制基因提供线索和热点位点.方法 选取1号染色体8个微卫星多态位点标志物,采用新鲜和石蜡组织基因组DNA抽提-PCR扩增-变性聚丙烯酰胺凝胶垂直电泳-银染法-全自动凝胶成像系统分析,检测80例浸润性导管癌及癌旁正常组织基因组DNA的杂合性缺失（LOH）频率.应用x2检验对实验结果进行综合分析.结果 80例浸润性导管癌中有45例（56.3％）至少在一个位点出现LOH,其中D1S1310微卫星位点频率最高,为35.7％（25/70）.结论 乳腺浸润性导管癌染色体1p36发生高频率LOH,提示1p36.23 ～ 33区间可能存在与乳腺癌发生、发展有关的抑癌基因.Objective The study of loss of heterozygosity （LOH） on chromosome 1p36 was performed to locate the deletion areas probably harboring tumor suppressor genes in invasive ductal breast carcinoma not otherwise specified （IDC NOS）.Methods Eighty paired breast cancer/normal tissue DNA samples were examined for LOH on chromosome lp36 using eight polymorphic microsatellite （MS） loci.The PCR products were electrophoresed on 8% denatured polyacrylamide gel and stained using silver staining.Finally,the data were analysed and compared with the clinicopathological parameters using statistical analysis.Results In 80 IDC NOS,LOH was identified in 45 cases （56.3 %） at least in one MS locus.MS locus D1S1310 showed the highest rate of LOH [35.7% （25/70）].Conclusion Chromosome 1p36 might be the highly deleted region.The results of this study indicate that the chromosomal regions 1p36.23-33 might contain tumor suppressor genes associated with human breast carcinomas.

关 键 词：癌 导管 乳腺 杂合子丢失 基因 肿瘤抑制 微卫星 1p36 

分 类 号：R737.9[医药卫生—肿瘤]