低豆粕用量下脂肪氧合酶好氧催化大豆油的反应研究  

Aerobic Catalyzed Lipoxygenation of Soybean Oil with Low Level Soymeal

作　　者：蔡燕[1] 周红波[1] 朱金丽[1] 

机构地区：[1]南通大学化学化工学院,江苏南通226019

出　　处：《大豆科学》2013年第5期683-686,共4页Soybean Science

基　　金：国家自然科学基金(21006054);南通大学引进人才科研启动基金(12R038)

摘　　要：为实现低量豆粕(m豆粕/m豆油=10%)高效催化氧化大豆油合成氢过氧化豆油,对此氧化反应进行了单因素优化,并探讨了添加表面活性剂和引入超声辐射对豆油催化效率的影响。结果表明:豆粕催化豆油的优化条件为:在15℃下,0.05 g豆粕溶于3.3 mL pH6的磷酸盐缓冲液后,逐滴加入0.5 g豆油和0.15 mL乙醇,通氧反应3 h后产率为12%。加入表面活性剂CTAB或β-环糊精后,反应产率呈现了不同程度的降低。超声辐射(100 W,40 Hz)提高了豆粕酶促催化氧化大豆油的效率,将反应的平衡时间减少为常规的1/3,将产率由12%提高到35%。The performance of low level soymeal （ m_soymeal/m_soybean oil = 10% ） in catalyzing the hydroperoxidation reaction using soybean oil as substrate was evaluated, and surfactants as well as ultrasonic wave were introduced to improve the lipoxygenation reaction yield. The optimal conditions for soybean oil were 0.5 g soybean oil in 3.3 mL phosphate buffer solution of pH6 with 0.15 mL ethanol as co-solvent reacting for 3 h at 15℃. Under those optimal conditions the yield reached 12%. The addition of surfactants such as CTAB and β-CD reduced the hydroperoxide yield. The employment of ultrasonic wave（ 100 W,40 Hz）during the aerobic catalysis reached equilibrium after 60 min with the yield of 35% ,which could both accelerate the enzymatic reaction and improve the reaction yield.

关 键 词：豆粕 脂肪氧合酶 大豆油 过氧化 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]