茜素与β-胡萝卜素自由基抗氧化和一氧化氮释放的研究  

Studies on the Antioxidant Effect and Release of Nitric Oxide of Alizarin and P-Carotene

作　　者：崔剑[1] 李兆陇[1] 洪啸吟[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《化学学报》2000年第11期1442-1446,共5页Acta Chimica Sinica

基　　金：教育部博士点基金;清华大学;香港浸会大学;北京中医大学联合基金

摘　　要：在均相体系中利用紫外吸收光谱法,研究了偶氮二异丁睛(AIBN)引发的亚油酸(LH)过氧化反应,发现茜素(Alz)和β-胡萝卜素(β-C)对LH过氧化均有抑制作用,并表现出协同效应.膨胀计法研究表明Alz和β-C对AIBN引发的苯乙烯自由基聚合有阻滞作用,发现Alz与β-C间存在明显的协同阻/缓聚作用.通过在培养的人脐静脉内皮细胞中的研究表明,Alz可促使内皮细胞释放一氧化氮(NO),其作用比乙酰胆碱(Ach)更为显著.Peroxidation of linoleic acid was initiated by azobis (isobutyronitrile) in ethanol and inhibited effectively by alizarin and β- carotene. It was found alizarin and β- carotene either alone or in combination exhibited obvious inhibiting effect, and the decay of β - carotene slowed down in the presence of alizarin. Quantitative kinetic measurement showed alizarin and β - carotene can act as free radical inhibitors for bulk free radical polymerization of styrene initiated by AIBN at 60℃. The experimental results indicated that their synergistic efficiency depended on the amount of alizarin and β- carotene used. The effect of alizarin and acetylcholine on endothelial cell release of nitric oxide in human umbilical vein was also observed. The alizarin exhibits a more efficiency release of nitric oxide than that of acetylcholine.

关 键 词：茜素 Β-胡萝卜素 抗氧化剂 一氧化氮 释放 中药 

分 类 号：O657.3[理学—分析化学]