慢性牙周炎患者口腔菌群结构分析  

Analysis the Oral Bacterial Diversity of Chronic Periodontitis

作　　者：吴江华[1] 徐洁[2] 马校卫[3] 兰鸿霞[3] 黄祖新[3] 

机构地区：[1]福建师范大学医院,福建福州350007 [2]福建省立医院,福建福州350001 [3]福建师范大学生命科学院,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2013年第6期125-130,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：农业部公益性行业科研专项(201303094)

摘　　要：运用限制性片段长度多态性(RFLP)方法检测分析慢性牙周炎患者龈下菌斑和唾液内细菌菌群结构及其分布.选取符合标准的慢性牙周炎患者的龈下菌斑和唾液为实验材料,通过构建16S rDNA基因文库,利用限制性内切酶AluⅠ对随机克隆进行酶切筛选,测定代表克隆的16S rDNA序列,测序结果进行聚类分析.结果表明:慢性牙周炎患者龈下菌斑细菌中厌氧菌属含量较多,慢性牙周炎患者龈下菌斑中检测到CP可能致病菌属,而唾液中未检测到.慢性牙周炎是以厌氧菌为主的混合感染性疾病,慢性牙周炎患者龈下菌斑可以作为稳定分析牙周致病菌的临床标本.Analysis the oral bacterial diversity of chronic periodontitis by restriction fragment length polymorphism （RFLP）. The bacterial 16S rDNA libraries were structured by the total DNA of dental plaque and saliva which sampled from the chronic periodontitis （CP） patients. And then, the restriction enzymes Alu I was emploied to screening two libraries. The operational taxonomic u- nits （OTUs） were sequenced and analysised by clustering analysis. The anaerobic bacteria was the mostnumerically abundant bacterium present in the dental plaque. The CP pathogenic bacteria was also found in the dental plaque, but it did not appear in the saliva. The CP is a infectious disease predominantly infected by anaerobic bacteria, the dental plaque can be used as clinical specimens for the research of CP pathogenic bacteria.

关 键 词：慢性牙周炎 龈下菌斑 16S RDNA 限制性片段长度多态性 

分 类 号：R781.4[医药卫生—口腔医学]