小麦新抗源抗锈性评价和SSR位点遗传多样性  

Evaluation of Resistance to Stripe Rust and Genetic Diversity Analysis by SSR Marker of Wheat Resources

作　　者：杨乐[1,2] 王琪琳[1,3] 曾庆东[1,3] 周新力[1,3] 康振生[1,3] 韩德俊[1,2] 

机构地区：[1]西北农林科技大学/旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100 [3]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《麦类作物学报》2013年第6期1305-1311,共7页Journal of Triticeae Crops

基　　金：国家高技术研究发展计划(863计划)项目(2012AA101503);国家公益性行业(农业)计划项目(201203014);高等学校学科创新引智计划资助项目(B07049)

摘　　要：为了解小麦条锈病新抗源兴资9104、品冬34、秦农142、彬旱355的抗病性及遗传基础,对其苗期和成株期分别进行抗条锈性鉴定,并以小麦全基因组SSR标记为工具分析了新抗源与四个感病材料(Avocet S、铭贤169、790-149-34、中国春)间的遗传多样性。结果表明,兴资9104、品冬34、秦农142、彬旱355对当前条锈病流行小种具有全生育期或成株期抗性;在975个SSR多态性位点共有3 390个等位性变异,每个位点平均等位变异3.48;全基因组和A、B、D基因组范围聚类结果基本一致,8个材料可分成三类,其中品冬34遗传背景与其他材料差异较大;随机抽取部分标记进行标记数量累加聚类,标记数量愈多,聚类结果愈稳定可靠。Exploiting diverse resistant germplasm resources and understanding their genetic base are helpful to material selection and strategy selection in wheat breeding. Four resistant cultivars, Xingzi 9104, Pindong 34, Qinnong 142 and Binhan 355, were used for assessment of resistance to stripe rust at both seedling and adult-plant stages. Meanwhile, nine hundred and seventy five polymorphic wheat SSR markers located in the whole genome were used to analyze the genetic diversity among them and 4 susceptible materials （Avocet S, Mingxian 169, 790-149-34 and Chinese spring）. Total 3 390 allelic variations were detected among the 8 testing cultivars, with a mean allele number of 3.48. The result of cluster analysis showed that the 8 cultivars could be classified into three groups in wheat whole ge- nome and A, B and D genome. The results indicated that Pindong 34 had the conspicuous diversity compared with others. On another hand, the result of clustering was proved to be more dependable with the increased number of randomly selected SSR markers.

关 键 词：小麦条锈病 抗源 SSR位点 遗传多样性 

分 类 号：S512.1[农业科学—作物学] S435.121.42