微卫星DNA与慢性阻塞性肺疾病易感性的研究  

A study on microsatellite DNA and susceptibility of chronic obstructive pulmonary disease

作　　者：于丽丽[1] 

机构地区：[1]赤峰学院附属医院检验科,内蒙古赤峰024000

出　　处：《国际检验医学杂志》2013年第21期2806-2807,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨微卫星DNA与慢性阻塞性肺疾病(COPD)易感性的关系。方法将64例COPD患者作为COPD组,以100例健康者作为对照组。采用聚合酶链反应(PCR)-短串联重复序列(STR)复合扩增技术对受检者的D18S51、D8S1179、D21S11、D7S820、CSF1PO、D3S1358、D5S818、D13S317、D16S539、D2S1338、D19S433、VWA、D12S391、D6S1043基因位点的遗传多态性进行分析。结果 COPD组患者D18S51-19、D7S820-9及D6S1043-17的阳性率分别为18.75%、25.00%及20.31%,均高于对照组,差异有统计学意义(P<0.05)。两组其他等位基因检出率的差异无统计学意义(P>0.05)。结论 D18S51-19、D7S820-9及D6S1043-17基因位点附近可能存在COPD的易感基因。Objective To explore the relationship of microsatellite DNA and susceptibility of chronic obstructive pulmonary disease（COPD）. Methods 64 patients with COPD were served as COPD group and 100 healthy people as control group. Polymerase chain reaction（PCR）-short tandem repeats（STR） multiplex PCR technique were employed to analyze genetic polymorphism of D]8S51, D8Sl179, D21Sll, D7S820, CSF1PO, D3S1358, D5S818, D13S317, D16S539, D2S1338. D19S433, VWA, D12S391 and D6S1043 genetic loci of the subjects. Results The positive rates of D18S51-19,D7S820-9 and D6S1043-17 of patients in COPD group were 18.75 %, 25.00 % and 20.31 %, respectively, which were all higher than those in control group, with statistically significant differenee（P〈0.05）. The difference of detection rates of other alleles in the two groups showed no significant（P〈0.05）. Conclusion There may be COPD susceptibility gene near the D18S51-19,D7S820-9 and D6S1043-17 genetic loci.

关 键 词：微卫星重复 肺疾病 慢性阻塞性 多态性 单核苷酸 疾病遗传易感性 

分 类 号：R563.9[医药卫生—呼吸系统]