白细胞介素-1β和肿瘤坏死因子在视网膜瞬间缺血重新灌注前后的定量分析  

Quantified analysis on retinal IL-1 β and TNFα in transient retinal ischemia reperfusion

作　　者：周跃华[1] 李志辉[1] 孙葆忱[1] 安伟丽[1] 

机构地区：[1]北京同仁医院眼科,100730

出　　处：《眼科研究》2000年第5期595-397,共1页Chinese Ophthalmic Research

摘　　要：目的观察瞬间性视网膜缺血重新灌注对大鼠视网膜白细胞介素 1β(IL-1β)和肿瘤坏死因子(TNFα)含量的影响。方法实验对象为21只(42眼)SD大鼠。以升高眼压的方法造成瞬间性视网膜缺血重新灌注的模型。视网膜内IL-1β和TNFα的含量用酶联免疫吸附测定(ELISA)的方法定量。结果正常的视网膜内含有少量的IL-1β和TNFα;视网膜缺血1min和5min组,视网膜内TNFα在缺血重新灌注的过程中与正常视网膜内的含量比较,均无显著性差异( P>0.05),但视网膜内的IL- 1β在缺血重新灌注后6h则比正常视网膜含量显著性增加( P<0.05)。结论瞬间性视网膜缺血重新灌注可以影响大鼠视网膜IL-1β和TNFα含量的改变,这种改变与视网膜缺血的程度有关。ObjectiveTo investigate the effec ts of transient retinal ischemia reperfus ion on the levels of retinal interleukin 1 β (IL 1β) and tumor necrosis factor α(TNFα).MethodsExperiments were perf ormed on 21 Sprague Dawley rats(42 eyes) .Retinal ischemia reperfusion was in duced by elevation of intraocular pr essure(IOP).The levels of retinal IL 1β and TNFα were quantified by enzyme li nked immunosorbent assays(ELISA) method. ResultsIn the control eyes,retinal IL 1β were 489 92±45 37pg/ml,and TNFα were 22 63±3 92pg/ml.In the treated eyes with 1 min of retinal ischemia,retinal IL 1β we re 538 37±23 05pg/ml,512 92±14 30pg/ml and 512 92±53 23pg/ml,and TNFα were 24 21±5 91pg/m l,23 42±8 16pg/ml and 19 16±2 17pg/ml at 6, 24,48h after reperfusion,respectively;I n the treated eyes with 5min of retinal ischemia,retinal IL 1β were 575 30±31 39p g/ml,Ｆ03 40±37 57pg/ml and 478 63±36 35pg/ml, and TNFα were 28 34±6 45pg/ml,22 65±8 89pg /ml and 19 12±2 64pg/ml,respectively.At 6 h after reperfusion,the levels of retina l IL 1β in the treated eyes with 1min o f retinal ischemia were significantly diff erent from those in the treated eyes wi th 5min of retinal ischemia,and they al l showed significantly different from those in the control eyes( P <0 05).The levels of retinal TNFα,however,were not signif icant difference from those in the contr ol eyes( P >0 05) at any time point in thi s study.ConclusionTransient retinal is chemia reperfusion can affect the levels of retinal IL 1β and TNFα,which is rel ated to the degree of retinal ischemia.

关 键 词：重新灌注 白细胞介素-1Β 视网膜缺血 TNFΑ 

分 类 号：R774[医药卫生—眼科]