青岛沿海大型海藻抑菌活性初探  

Preliminary study on the microbial inhibition activities of the ethanol extracts of the seaweeds in Qingdao coastline

作　　者：孙好芬[1] 奚洵[1] 李淑清[1] 赵健[1] 赵静[1] 徐爱玲[1] 夏岩[1] 

机构地区：[1]青岛理工大学环境与市政工程学院,青岛266033

出　　处：《青岛理工大学学报》2013年第5期50-54,共5页Journal of Qingdao University of Technology

基　　金：山东省自然科学基金资助项目(ZR2011EEQ026);青岛理工大学泰山学者建设工程专项经费资助项目

摘　　要：采用滤纸片法以金黄色葡萄球菌、大肠埃希氏杆菌、苏云金芽孢杆菌和枯草芽孢杆菌4株细菌和花生菌核菌、西瓜枯萎菌、小麦赤霉菌、马铃薯早疫菌、番茄早疫菌5株植物病原真菌作为受试菌株进行抗菌实验,对采自青岛沿海10种常见大型海藻乙醇提取物不同极性部位进行抑菌活性研究.结果表明,多管藻各组分的抑菌活性优于其他所选藻类,且其Fr.4组分对供试菌株均表现抑制活性.利用柱色谱分离以及现代波谱结构鉴定技术对Fr.4中活性物质进行初步研究,得到抑菌活性较高的单体化合物3-溴-4,5-二羟基苯甲醛.The antibacterial activities against the staphylococcus aureus, escherichia coli, ba- cillus thuringiensis, and bacillus subtilis （Ehrenberg） Cohn, as well as antifungal activities against five pathogenic fungi sclerotinia arachidis Hnzawa, fusariurn o：rysporurn f. sp. , sphacrales gibberella zeae , alternaria solani , and alternaria solani of ten kinds of marine algae growing in Qingdao＇s coastline are studied using disk diffusion method. In the microbi- al inhibition activity assays, the sub-fraction of ethanol extracts of polysihonia urceolata （Lightf.） Grey displays obvious activities. Among them, Fr. 4 exhibits the most attracting anti-microbial activity against all the tested strains. The subsequent isolation of the bioactive compounds from Fr. 4 by repeated column chromatography （CC） and spectroscopic methods leads to the identification of 3-bromo 4, 5-dihydroxybenzaldehyde, which displays a potent anti-microbial activity.

关 键 词：滤纸片扩散法 植物病原菌 海藻 抑菌活性 分离纯化 

分 类 号：X173[环境科学与工程—环境科学]