CD152基因多态性与儿童特发性脑卒中的关系  

CD152 gene polymorphisms in idiopathic childhood ischemic stroke

作　　者：王建军[1] 邹丽萍[2] 李久伟[3] 黄越[1] 雷媛[1] 

机构地区：[1]川北医学院附属医院儿科,四川南充637000 [2]中国人民解放军总医院儿科,北京100853 [3]北京儿童医院神经康复中心,北京100045

出　　处：《临床儿科杂志》2013年第11期1011-1014,共4页Journal of Clinical Pediatrics

摘　　要：目的探讨CD152启动子-1722T/C、-1661A/G以及外显子1+49A/G基因多态性与儿童特发性脑卒中发生的关系。方法 51例特发性脑卒中患儿作为病例组,74名健康汉族儿童作为对照组,采用PCR-RFLP方法进行CD152基因型分析。结果对照组和病例组不同CD152基因型分布经χ2检验符合Hardy-Weinberg平衡定律。CD152-1722 T/C、-1661A/G不同基因型及等位基因频率在病例组与对照组之间的差异无统计学意义(P均>0.05);病例组的CD152+49GG基因型及G等位基因频率较对照组明显升高,差异有统计学意义(χ2=7.053、6.351,P<0.05)。CD152+49A/G基因型频率与常见临床表型(如偏瘫、言语障碍、惊厥、意识改变以及发病前感染)之间无相关性(P均>0.05)结论 CD152+49A/G基因多态性可能与特发性卒中发生风险有关。Objectives To explore polymorphisms of CD152 gene promoters （-1722T/C,-1661A/G） and exon1 （＋49A/G） in children with idiopathic ischemic stroke and assess the association between these polymorphisms with the disease. Methods Us-ing the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method, polymorphisms in the CD152 exon1 region （＋49A/G） and promoter regions （-1722T/C,-1661A/G） were genotyped in 51 Han children with idiopathic ischemic stroke and 74 healthy Han children. Results The CD152 genotypes in control group and idiopathic ischemic stroke children were consistent to principle of Hardy-Weinberg Equilibrium （HWE） byχ2 test. The CD152＋49GG genotypes and G allele frequency in the patients showed a signiifcant increase compared to the controls （χ2=7.053, 6.351, P〈0.05）. However, the CD152-1722T/C,-1661A/G genotypes and allele frequency showed no signiifcant difference between 2 groups （P〉0.05）. Clinical features （hemi-paresis, dysarthria, seizures, consciousness change, and preceding infections） did not show signiifcant correlation with genotypes and frequency of the CD152＋49 polymorphisms in children with idiopathic ischemic stroke （P〉0.05）. Conclusions The CD152＋49A/G polymorphisms may relate to idiopathic ischemic stroke.

关 键 词：特发性卒中 CD152 单核苷酸多态性 儿童 

分 类 号：R743.3[医药卫生—神经病学与精神病学]