几种油脂对高脂饲料诱导的C57BL／6J肥胖小鼠脂代谢的影响  

Effects of oils on lipid metabolism in obese C57BL/6J mice induced by a high fat diet

作　　者：杨帆[1] 张永[1] 徐庆[1] 李瑞生[2] 杨雪艳[1] 刘英华[1] 王觐[1] 于晓明[1] 薛长勇 

机构地区：[1]解放军总医院营养科,北京100853 [2]解放军第302医院动物实验中心

出　　处：《卫生研究》2013年第6期901-906,914,共7页Journal of Hygiene Research

基　　金：国家自然科学基金(No.81172667)

摘　　要：目的观察几种油脂对高脂肪饲料诱导的C57BL／6J肥胖小鼠脂代谢的影响。方法选择4～5周龄C57BL／6J雄性小鼠J75只，随机分为5组，每组15只，分别喂饲含2％大豆油、中链甘油三酯（MCT）、花生油、橄榄油和茶油的高脂饲料，喂养12周，观察和比较各组小鼠体重、体脂、饲料消耗量、血脂和脂肪组织脂代谢相关酶以及白色脂肪组织和肝脏病理变化。结果实验结束时，MCT、花生油、橄榄油及茶油组小鼠的体重、体脂重均显著低于大豆油组（P〈0．05）。MCT组血清TG、TC、LDL．C和肝脏TG、TC显著低于大豆油组（P〈0．05），白色脂肪组织中cAMP、PKA、HSL和ATGL显著高于大豆油组（P〈0．05）。MCTr、橄榄油和茶油组小鼠肝脏未见脂肪浸润，而大豆油和花生油组可见明显的脂肪浸润。结论与大豆油相比，MCT、花生油、橄榄油及茶油均可降低肥胖小鼠的体重和体脂，MCT还能降低血清TG、TC、LDL-C和促进白色脂肪组织的脂肪动员；在改善血脂方面，橄榄油和茶油的作用没有MCT明显，但与大豆油和花生油相比不会导致明显的脂肪肝。Objective To investigate and compare the effects of oils on lipid metabolism in obese C57BL/6J fed a high fat diet. Methods 75 male C57BL/6J mice （4-5 weeks old） were used and randomly divided into 5 groups, 15 mice in each group, and were fed a high fat diets with 2% soybean oil, medium-chain triglyceride （MCT）, peanut oil, olive oil and tea seed oil respectively for 12 weeks. Body weight, body fat, diet intake, blood lipid profiles and enzymes relevant to lipid metabolism in white adipose tissue（WAT） as well as pathologic changes in WAT and livers from all groups were observed and compared. Results At the end of study, the body weight and body fatweight were significantly lower in MCT, peanut oil, olive oil, tea oil groups than in soybean oil group（P 〈 0.05）. The mice in MCT group showed significantly lower TG, TC, LDL-C in serum and lower TG, TC in liver than those in soybean oil group （P 〈 0.05）. The cAMP, PKA, HSL, ATGL in WAT in MCT group showed higher than those in soybean oil group （ P 〈 0.05 ）. There was no fatty infiltration in the livers of mice fed MCT, olive oil and tea oil group, but visible fatty liver in soybean oil and peanut oil group were found. Conclusion Compared to soybean oil, MCT, peanut oil, olive oil and tea oil could reduce body weight and body fat weight in obese mice fed a high fat diet, MCT also decreased TG, TC, LDL-C in serum and promote lipid mobilization in WAT. As to improving blood lipids, olive oil and tea oil were less obvious than MCT was, and both oils did not induce significant fatty liver when compared with soybean oil and peanut oil.

关 键 词：中链甘油三酯 油脂 体重 体脂 甘油三酯 

分 类 号：R151.2[医药卫生—营养与食品卫生学] Q591[医药卫生—公共卫生与预防医学]