高效液相色谱法测定食品接触聚苯乙烯制品中9种荧光增白剂  

Determination of Nine Fluorescent Whitening Agents in Food Contact Polystyrene Materials by High Performance Liquid Chromatography

作　　者：吴钟玲 陈树东 林晓佳 穆同娜[2] 吴治将[3] 郭新东 郭茂章 冼燕萍 罗东辉 

机构地区：[1]广州市质量监督检测研究院,国家加工食品质量监督检验中心(广州),广东广州510110 [2]北京市海淀区产品质量监督检验所,北京100094 [3]顺德职业技术学院,广东顺德528000

出　　处：《现代食品科技》2013年第11期2757-2761,共5页Modern Food Science and Technology

基　　金：国家自然科学基金(No 51246006);国家质检总局科技计划项目(20120K074)

摘　　要：建立了高效液相色谱-荧光检测法（HPLC-FLD）同时测定食品接触聚苯乙烯（PS）塑料制品中9种荧光增白剂（FWA52、FWA135、FWA184、FWA185、FWA199、FWA367、FWA368、FWA378、FWA393）的方法。样品以三氯甲烷溶液提取后，以甲醇沉淀塑料制品中的聚合物，以Phenomenex Gemini C18色谱柱（4.6 mm×250 mm，5 μm），甲醇和水作为流动相进行梯度洗脱，有效分离了9种目标物质。考察了方法的灵敏度、线性范围、回收率和精密度，结果表明，9种荧光增白剂在0.32~200 μg/L范围内线性关系良好，相关系数均大于0.9992，方法定量限（S/N=10）在1.0~8.0 μg/kg之间，添加水平为5~500 μg/kg时，样品的回收率为88.0%~98.9%，相对标准偏差（RSD，n=6）在2.11~4.34%之间。该法前处理简单、回收率高、精密度好，适用于食品接触聚苯乙烯（PS）塑料制品中9种荧光增白剂的测定。A new analytical method was developed for the simultaneous determination of nine fluorescent whitening agents （FWA52, FWA135, FWA184, FWA185, FWA199, FWA367, FWA368, FWA378 and FWA393） in food contact polystyrene materials by high performance liquid chromatography-fluorescence detector （HPLC-FLD）. The sample was extracted with trichloromethane, and then cleaned up by precipitating the polymer with methanol and analyzed by HPLC using a Phenomenex Gemini C18 column with methanol - water as the mobile phase for gradient elution. The result indicated that the limits of quantification （LOQs） of nine FAWs were ranged from 1.0~8.0 μg/kg and the linear correlation coefficients （r〉0.9992） were obtained within the irrespective linear ranges （0.32~200 μg/L）. The recovery rates from spiked samples at the spiking levels of 5~500 μg/kg were ranged from 88.0% to 98.9% with RSD of 2.11~4.34% （n=6）. The method was simple, precise and had high recovery rate for determination of FAWs in food contact polystyrene materials.

关 键 词：荧光增白剂 高效液相色谱法 聚苯乙烯 食品接触材料 

分 类 号：TS206.4[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]