喉鳞癌中p53基因的检测及其意义  

Detection of Mutation of p53 Gene in Exon 7 in Laryngeal Squamous Cell Carcinoma by Silver Staining PCR-SSCP

作　　者：刘世喜[1] 张晓晴[2] 梁传余[1] 王力红[1] 林代诚[1] 

机构地区：[1]华西医科大学附属第一医院耳鼻咽喉科,成都610041 [2]华西医科大学附属第四医院耳鼻咽喉科

出　　处：《华西医科大学学报》2000年第4期497-499,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金!(批准号 395 0 0 16 0 );美国纽约中华医学基金会专项基金

摘　　要：为了探讨喉鳞癌中 p5 3基因与肿瘤临床分期、病理分化程度及淋巴结转移的关系 ,应用 PCR- SS-CP银染系统检测 6 0例喉鳞癌新鲜组织中抑癌基因 p5 3基因第 7外显子的突变情况。结果 :p5 3基因第 7外显子突变 2 5例 ,突变率为 41.7% .临床 ～ 期突变率为 2 6 .9% (7/ 2 6 ) , ～ 期突变率为 5 2 .9% (18/ 34) ;高、中、低分化程度分布为 2 3.5 % (4 / 17)、37.5 % (9/ 2 4)、6 3.2 % (12 / 19) ;无淋巴结转移者为 2 8.1% (9/ 32 ) ,有淋巴结转移者为5 7.1% (16 / 2 8) ;差异均有显著意义 (P<0 .0 5 )。结论 :提示 p5 3基因第 7外显子突变与喉鳞癌临床分期、病理分化程度及肿瘤颈淋巴结转移密切相关。The objective of this work was to detect the mutation of p53 gene in exon 7 in fresh tumor samples of laryngeal squamous cell carcinoma (LSCC). Polymerase chain reaction (PCR) and silver staining single strand conformation polymorphism (SSCP) were used. From among 60 patient samples, 25 showed an extra band in SSCP. The mutation rate was 41.7%. The mutation rate for patients in clinical stages Ⅲ and Ⅳ was 52.9%, which was significantly higher than that for patients in stages clinical Ⅰ and Ⅱ. The mutation rates were 23.5%, 37.5% and 63.2% in the well, moderately and poorly differentiated LSCC, respectively. The mutation rate for the patients with metastasis was 57.1%, which was significantly higher than that of the patients without metastasis. These results suggest that the mutation of p53 gene in exon7 be closely related with the clinical stages, differentiation and neck lymphatic metastasis of laryngeal carcinoma.

关 键 词：P基因 喉鳞癌 基因突变 淋巴结转移 

分 类 号：R739.6[医药卫生—肿瘤]