三磷酸腺苷生物发光法在卵巢癌体外化疗药物敏感试验中的应用  

Application of an ATP-Bioluminescence Assay in Ovarian Cancer Chemosensitivity Testing in Vitro

作　　者：楼江燕[1] 彭芝兰[1] 刘珊玲[1] 王和[1] 

机构地区：[1]华西医科大学附属第二医院妇产科,成都610041

出　　处：《华西医科大学学报》2000年第4期553-556,共4页Journal of West China University of Medical Sciences

基　　金：高等学校博士学科点专项基金

摘　　要：为探讨新鲜卵巢癌标本化疗药物敏感试验结果与临床疗效的相关性 ,采用三磷酸腺苷生物发光法(ATP- CVA) ,用液闪计数仪对 2 5份新鲜卵巢癌组织标本进行药敏检测。结果显示 :在 ATP浓度为 10 - 9～ 10 - 5mol/ m l时 ,ATP标准品与发光计数值呈一线性关系 ,Y=1.48X+ 15 .0 3,相关系数为 0 .996 3;所检测的 2 5份标本中成功 2 2份 ,试验的可评价率为 88% ,其敏感性为 85 .7% (12 / 14) ,特异性为 83.3% (5 / 6 ) ,阳性预测值为 92 .3%(12 / 13) ,阴性预测值为 71.4% (5 / 7) ,总的预测准确率为 85 % (17/ 2 0 )。上述结果表明 ,本药敏试验结果的预测准确性较高 ,与临床疗效之间具有很好的相关性 ,值得进行更深入的研究。This study was aimed to make an evaluation of ATP bioluminescence assay (ATP CVA) in ovarian cancer chemosensitivity testing in vitro and to analyze the correlation between in vitro results and in vivo response. ATP CVA was used to determine the drug sensitivity of 25 specimens of fresh ovarian cancer cells. The ATP levels of drug exposed tumor cell cultures were compared with those of control cultures, and based on the percentage decrease in ATP levels, drug sensitivity was evaluated. A decrease of >70% was defined as strong sensitivity (SS),50% 70% as partially sensitivity (PS),<50% as resistance (RR). Patients' responses were obtained by accepted clinical assessment methods after six treatment courses. The results showed that in a range of 10 -9 10 -5 mol/ml,there was a linear relationship between the log ATP and the log luminescence,Y=1.48X+15 03, correlation coefficient r =0.9963. In this study 25 fresh specimens of ovarian cancer were subjected to ATP chemosensitivity assays. 22 assays were completed successfully, yielding an evaluability rate of 88% (22/25). The correlation between clinical responses and in vitro data on chemosensitivity was evaluated retrospectively. The sensitivity was 85.7%, specificity 83.3%, positive predictive value 92.3%, negative predictive value 71.4%, and the total predictive accuracy 85%.This study demonstrated a good correlation between in vitro results and in vivo response. It is worth further clinical investigation.

关 键 词：卵巢癌 药敏试验 三磷酸腺苷生物发光法 

分 类 号：R737.31[医药卫生—肿瘤] R73－361[医药卫生—临床医学]