β-1,3-葡甘露聚糖酶辅助提取圆红冬孢酵母油脂  

β-1,3-glucomannanase assisted lipid extraction from Rhodosporidium toruloides

作　　者：靳国杰[1,2] 杨晓兵[1,2] 沈宏伟[1,3] 王雅南[1,2] 龚志伟[1,2] 赵宗保[1,3] 

机构地区：[1]中国科学院大连化学物理研究所生物技术部,辽宁大连116023 [2]中国科学院大学,北京100049 [3]大连洁净能源国家实验室(筹)生物能源研究部,辽宁大连1160233

出　　处：《生物工程学报》2013年第11期1581-1589,共9页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展计划(973计划)(No.2011CB707405);中科院知识创新工程重要方向项目(No.KSCX2-EW-G-1-3)资助~~

摘　　要：为考察β-1,3-葡甘露聚糖酶辅助提取圆红冬孢酵母Rhodosporidium toruloides油脂工艺的应用潜力,以R.toruloides Y4发酵醪液为原料,乙酸乙酯为溶剂,在10 L规模下研究了酶解和萃取条件对油脂提取率的影响,并进行了初步物料平衡分析。结果表明:酶处理0.5 h,油脂提取率可达71.1%,酶解效率与10 mL小体系所取得的结果相当。通过多次萃取,有效缓解了乳化现象对油脂提取的影响。经过3次萃取,油脂提取率、溶剂回收率和总物料回收率分别达92.9%、87.0%和94.2%。酶辅助提取油脂工艺可直接利用油脂发酵醪液,提取率高,对设备要求低,具有很好的工业化应用潜力。To evaluate the effectiveness of enzymatic assisted extraction （EAE） of lipid from the oleaginous yeast Rhodosporidium toruloides in the presence of β-1,3-glucomannanase at a larger scale, we investigated the effects of enzymatic treatment and extraction conditions on lipid extraction yields at 10-L scale by using the broth ofR. toruloides Y4 as the feed and ethyl acetate as the solvent. When it was treated for 0.5 h, the lipid extraction yield reached 71.1%, indicating that the enzymatic treatment process reached similar efficiency to that obtained at 10-mL scale. The inhibitory effect of emulsification was greatly reduced by repeated extraction. After extracted for three times, yields of lipid extraction, solvent recovery and total material recovery reached 92.9%, 87.0% and 94.2% respectively. As it can use the lipid production slurry with good extraction efficiency, EAE technology is promising for industrial production of microbial lipids.

关 键 词：微生物油脂 油脂提取 酶辅助 圆红冬孢酵母 放大 生物柴油 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]