不同剂量的食用白酒对大鼠心脏结构与功能影响的研究  

Effects of different dose of edible liquor on cardiac structure and function in rats

作　　者：刘爱东[1] 蒋慧[1] 牛晨[1] 田虹[1] 潘贵书[1] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563000

出　　处：《重庆医学》2013年第32期3926-3928,共3页Chongqing medicine

基　　金：贵州省科技厅资助项目(黔科合重大专项字[2010]6004号);贵州省遵义市科技局资助项目(遵市科合社重字[2009]1号)

摘　　要：目的观察不同剂量的食用白酒对大鼠心脏结构和功能的影响,并探讨其对心脏损害的机制。方法将成年雄性SD大鼠54只分成生理盐水组、乙醇组和白酒组,每组各分为低、中和高剂量组。灌胃12周后,监测大鼠左心室内压,计算左心室内压变化幅度;测定全心质量指数;快速Masson染色观察心肌细胞纤维化情况;酶联免疫吸附试验(ELISA)检测心肌组织血管紧张素Ⅱ(AngⅡ)含量;荧光定量聚合酶链反应(PCR)法检测心肌组织血管紧张素转换酶(ACE)、ACE2mRNA表达。结果白酒、乙醇增加了全心质量指数、左心室内压变化幅度,剂量依赖性地加重了心肌细胞变性,纤维组织增生,且剂量依赖性地增加了心肌组织AngⅡ含量及ACE mRNA表达。低剂量的白酒、乙醇增加了心肌组织ACE2mRNA表达,但其中、高剂量降低了心肌组织ACE2mRNA表达。结论长期饮酒可破坏心肌内的ACE和ACE2平衡,使ACE和AngⅡ升高,ACE2降低,导致心肌纤维化,其纤维化的变化程度与饮酒量以及时间长短有关。Objective To investigate the effects of different dose of edible liquor on cardiac structure and function in rats,and explore its underlying mechanisms. Methods 54 male SD rats were randomly divided into saline group,alcohol group,liquor group, each group were further divided into low-, middle-and high-dose groups. After 12 weeks of feeding, left ventricular pressure was de- termined,and left ventricular pressure amplitude and indexes of HW / BW were calculated. Myocardial fibrosis was observed by Masson staining, Ang Ⅱ content of myocardial tissue were detected by ELISA,and ACE and ACE2 mRNA expressions were detec- ted by RT-PCR,respectively. Results Left ventricular pressure amplitude and HW/BW index were increased by both liquor and al- cohol. The myocardial cell turbidity, degeneration, hyperplasia of fibrous tissue change were gradually worsened, and the Ang Ⅱ con tents and the ACEmRNA expressions of the myocardial tissue were also increased by both liquor and alcohol. The ACE2 mRNA ex- pression of the myocardial tissue were higher by low-dose liquor and alcohol,while the ACE2 mRNA expression of the myocardial tissue were lower by middle and high-dose liquor and alcohol. Conclusion Long-term alcohol consumption can damage the balance of cardiac ACE and ACE2 ,causing the increase of ACE, Ang Ⅱ and the decrease of ACE2, resulting in myocardial fibrosis, whose degree is related to the amount of alcohol consumption and drinking history.

关 键 词：血管紧张素Ⅱ 肽基二肽酶A 心肌组织 食用白酒 

分 类 号：R363[医药卫生—病理学]