单峰驼凝乳酶原的原核表达和活性检测  

Prokaryotic expression of dromedary(Camelus dromedarius) prochymosin and its activity detection

作　　者：普燕[1] 李轶杰[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《中国乳品工业》2013年第11期12-15,共4页China Dairy Industry

基　　金：新疆自治区动物学重点学科-乳品工程资助(2011001)

摘　　要：骆驼凝乳酶具有独特的凝乳特性,凝乳酶活性高。通过原核表达系统高效表达获得单峰驼(Camelus dromedarius)凝乳酶原,经酸化/中和处理后,活化的单峰驼凝乳酶能够凝固新鲜牛乳与双峰驼乳。同时单峰驼凝乳酶原的活化条件表明,酸化pH值必须低于4.5,活化时加入NaCl能显著提高单峰驼重组酶原的自剪切效率,研究结果为单峰驼凝乳酶特性的深入研究奠定了实验基础。Camel chymosin has the unique characteristic and the high clotting activity. In this study, the dromedary prochymosin was obtained in a effective prokaryotic expression system,after acidification and neutralization treatment, the activated camel chymosin can clot the flesh bovine and bactrian camel milk Respectively. At the same time, the conditions of dromedary prochymosin activation showed, the pH must be below 4.5 during the prochymosin activation process, and adding NaC1 can improve the efficiency of self-cleavage. These results could pro- vide some experimental basis for further study on the characteristics of camel chymosin.

关 键 词：单峰驼 凝乳酶原 原核表达 凝乳 活性检测 

分 类 号：Q814[生物学—生物工程]